What You Will Achieve
Exciting opportunity to join an innovative group at the nexus of functional genomics, new molecular biology techniques and target discovery/exploration within Oncology. Integrative Biotechnologies (IBT) is a collaborative group that applies expertise in cell engineering, proteomics and functional genomics across Pfizer’s diverse Oncology portfolio to translate novel breakthroughs into therapies that change patients’ lives. This position is located at Pfizer’s La Jolla CA campus and within a team focused on identifying and utilizing cutting-edge technology and functional genomics to advance drug development. Team members pursue tumor and immune cell targets while leveraging diverse therapeutic modalities that include enzymatic inhibitors and protein degraders.
We are seeking an experienced cell and molecular biologist to contribute to the success of the IBT group. Responsibilities include using CRISPR-based methods for modification or disruption at scales ranging from individual genes up to genome-wide pooled screens. Specific skills that are highly desired include excellent cell culture technique with experience using lentiviral gene delivery, expertise in PCR-based methods and preparation of NGS libraries. Successful candidates will have a team focused attitude that helps build and strengthen collaborations with colleagues across Oncology Research & Development. Please include a cover letter with your application.How You Will Achieve It
Carry out hands-on lab work for target discovery, validation and characterization using genetic and chemical approaches in cultured and primary cells.
Develop and engineer cell models in alignment with needs of the drug discovery team.
Complete pooled CRISPR screens to uncover novel genetic connections to drug response and resistance.
Identify and optimize new methods and workflows to help advance the drug discovery pipeline
Communicate science and strategy effectively with oral and written formats.
M.S. with 2+ years of experience or B.S. with 4+ years of experience, with degree in Genetics, Molecular Biology or a related discipline.
Prior experience with gene targeting methods (for example CRISPR/shRNA/siRNA).
Extensive cell culture experience with proficiency culturing and maintaining many cell lines and/or flasks simultaneously
Substantial experience in design, execution, and interpretation of cell culture-based experiments.
Strong ambition and creative problem-solving abilities to achieve data-driven decisions.
Excellent written and oral communication skills.
Experience with a variety of molecular biology and biochemistry techniques (DNA/RNA extraction, Western blot, qPCR, immunoprecipitation, cloning and flow cytometry).
Hands-on experience using laboratory automation, e.g. Incucyte, plate readers, FACS, and/or liquid-handling robotics.
Proficiency using Spotfire, DepMap/CCLE, UCSC Genome Browser, and/or SnapGene.
OTHER JOB DETAILS
Pfizer requires all U.S. new hires to be fully vaccinated for COVID-19 prior to the first date of employment. As required by applicable law, Pfizer will consider requests for Reasonable Accommodations.
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EEO & Employment Eligibility
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.
Research and Development