A Postdoctoral Research Fellowposition is available in the Mass Spectrometry Technology group in Drug Safety R&D Andover, MA for a highly motivated and innovative Ph.D. graduate. The candidate will utilize the entire suite of cutting-edge mass spectrometry technologies to elucidate changes in proteome phosphorylation in response to drug treatment. The newly established approaches will lead to the implementation of highly-plexed phospho-peptide assays for relevant signaling pathways across different therapeutic modalities. This offers a platform compatible with the use of chemical biology for target engagement and spatial resolution proteomics techniques. This role is within the state-of-the-art mass spectrometry lab in Global Discovery, Investigative and Translational Sciences (G-DITS) organization and brings multidisciplinary opportunities across in Drug Safety R&D and Pfizer. The expectation is that this work will lead to publications in top-tier journals, enable the candidate to gain a broader knowledge in drug development and toxicology discipline, and set the foundation for a career in industry or academia. This position requires a strong background in biochemistry, hands-on experience in mass spectrometry-based proteomics and demonstrated leadership in emerging technologies and innovation.
- The successful candidate will be responsible for the development of targeted (PRM, SureQuant) and unbiased mass spectrometry to affinity enrich and elucidate peptide phosphorylation.
- Ability to implement highly-plexed assays for selected signaling pathways using hiRes MS (Thermo Exploris 480 and Eclipse Tribrid).
- Deliver kinase activity profiling platform compatible with affinity-/activity-based approaches and spatial resolution techniques such as phosphoproteome analysis from FFPE sections isolated by laser capture microdissection.
- Demonstrate the utility of the developed methodologies by their application for hypothesis generation and testing of the mechanism of toxicities.
- Participate in all phases of mass spectrometry laboratory operations including technology evaluations, development of sample preparation and separation methods, maintenance and troubleshooting of chromatography and mass spectrometers.
- Apply analytical mindset, strong problem-solving, and trouble-shooting skills to enable technology and scientific advancements.
- Ensure all tasks and responsibilities are carried out according to scientific and ethical standards e.g., responsible for electronic notebook record-keeping, and raw data storage.
- Publications in peer-reviewed scientific journals and external/internal presentations.
- Qualified applicants must hold a Ph.D. in Analytical Sciences, Biochemistry, or closely related fields.
- Proficient with protein biochemistry, expertise in biological sample handling, and application of complex sample preparation methodologies applied to protein mass spectrometry.
- Expertise in hiRes MS, nanoLC and relevant software packages for data analysis.
- Strong written and oral communication skills, and interpersonal skills for collaboration.
- Well organized, self-motivated, ability to multitask and with attention to detail.
- Strong scientific curiosity and interest in drug development and technology innovation.
- Understanding of enrichment strategies using IMAC, TiO2, click-chemistry.
- Strong motivation for emerging technologies for single-cell proteomics.
- Record of peer-reviewed publications.
- R, Python or similar programming experience
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Eligible for Relocation Assistance
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Research and Development