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Senior Research Associate I

Employer
BioSpace Recruitment Services
Location
92121, San Diego
Start date
Sep 12, 2022

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Description
This position is responsible for transient mammalian expression of recombinant proteins and generation, maintenance, and scale-up of stable cell lines. The applicant should be self-motivated, able to work with some autonomy, be adaptable and highly attentive to details, demonstrate strong analytical and organizational skills, and possess excellent written and verbal communication skills.

Essential Functions:

  • Transient transfection of HEK293 and CHO cells for routine recombinant protein production.
  • High-throughput transient monoclonal antibody (mAb) expression from 96/24-well plates to large shake-flask cultures.
  • Stable cell line generation, single-cell cloning, scale up & maintenance (including but not limited to HEK293 and CHO) for recombinant protein and antibody production.
  • Stable cell line generation, single-cell cloning, scale up & maintenance (including but not limited to HEK293 and CHO) for cell surface target expression.
  • Large-scale fed-batch culture of stable CHO clones and pools to provide gram-scale quantities of mAbs for further analysis (including but not limited to shake flasks, WAVE bag and benchtop bioreactor systems).
  • Routine cleaning and maintenance of incubator shakers and biosafety cabinets.
  • Summarizes experimental data and present findings at internal project and department meetings and recommends next steps.
  • Maintain organized and accurate records/lab notebooks.
  • Follow and adhere to SOPs and laboratory safety regulations.
  • Independently solve problems, troubleshoot equipment, optimize and implement procedures and protocols that improve experimental throughput.
  • Participate in multi-departmental projects and communicate results to project and group leaders clearly and effectively.
  • Assists junior colleagues in troubleshooting.
  • Contribute to the general laboratory environment, acting as a good steward/citizen, suggesting, and promoting good laboratory policies.
  • May write sections of research study reports, manuscripts, or patent applications.

 

Competencies:

  • Capacity to organize and complete multiple assignments, of varying priorities, while working to deadlines.
  • Ability to plan ahead, anticipate bottlenecks and potential risks, and then communicate accordingly.
  • Ability to communicate clearly, and to report and present experimental results and analysis to department colleagues.
  • Team player, intensely committed to the success of the team/project, and getting the job done well in a highly dynamic environment.
  • Ability to juggle multiple tasks of varying priorities and to function in a fast-paced entrepreneurial environment.
  • Ability to work independently and perform experiments to a very high standard.
  • Self-motivated, with a results-oriented outlook, adaptable, and highly attentive to details with strong analytical and organizational skills, and excellent interpersonal skills, including written and verbal communication skills.
  • Strong analytical and organizational skills.


Required Education & Experience:

  • Bachelors' degree in Biological Sciences (or related degree) and least 5+ years related experience, or Masters' degree and 3+ years of experience.
  • Preference will be given to candidates with experience in stable cell line generation, mammalian cell culture and bioproduction.
  • Experience within the field of antibody research is a plus.
  • Experience with CHO stable cell line generation & screening for optimal expression by ELISA is a plus.
  • Experience with other cell types (bacterial, yeast, baculovirus) for protein production is a plus but not required.

 

Knowledge Requirements:

  • Strong scientific knowledge of recombinant protein expression
  • Mammalian cell culture and cell transfection methodologies
  • Proficiency working with Microsoft Word, Excel, and PowerPoint
  • Proficiency with sequence analysis software is preferred, including sequence alignments, structure prediction and similarity searches.
  • Ability to work with numerous samples in parallel while following detailed scientific protocols and maintaining meticulous scientific record keeping.
  • Basic laboratory techniques
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