Scientist I-Virologist

Scientist I-Virologist

Job ID: req2827
Employee Type: exempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc.  The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION

Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Applied and Developmental Research Directorate, consists of three sections (Basic Research, Clinical/Translational Research, and Bioinformatics) and has investigated HIV pathogenesis. We have been studying the mechanism of drug resistance and viral fitness in people living with HIV/AIDS (PLWHA) and  Innate immune responses against RNA or DNA virus infection under contract with NIAID/NIH. Our research aims to understand the mechanism of HIV infection and persistent immune activation during antiretroviral therapy and develop novel therapies to support NIAID clinical studies for PLWHA.

Recently LHRI has started a collaboration with NIAID for analysis of the function of defective proviral HIV (DOI: 10.1073/pnas.1917876117, DOI: 10.1073/pnas.1609057113) and seeks a productive and creative scientist who has worked on  HIV or RNA virus infection assay and T cell immunology to conduct the defective proviral study. 

KEY ROLES/RESPONSIBILITIES

• Characterization of molecular mechanism of the persisting provial HIV using multiples techniques ( tissue culture, WB, Immune precipitation, Ziss confocal microscope, AMNIS, Isoplexis, 10xGenomic Chromium, Singe-cell RNA-Seq system, RNA-seq using Nextseq 2000,  SeqStudio, QuantStudigo Absolute Q DNA digital PCR, RNA scope, Quaterex SR-X Biomarker Detection System, construction expression plasmids, knockdown or knock-in using siRNA or CRISPR).
• Publishing paper(s) and presenting the findings in international or domestic scientific conference(s)

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

• Possession of a PhD degree from an accredited college or university (according to CHEA). (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
• Two (2) years postdoctoral (PDF) experience.
• Publications with least one first authorship during PDF.
• Ability to obtain and maintain a security clearance.
  
  

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration: 

• The candidate needs the expertise in handling the infectious virus.
• Knowledge of the function of HIV proteins, DNA or RNA recombination.
• RNA handling, including in vitro RNA transcription and RNA transfection,
• Gene cloning, construction of expression vectors and gene cloning, other molecular biology skills, and cell culture (primary T cells and cell lines).
  

JOB HAZARDS

• This position is subject to working with or have potential for exposure to infectious
  material, requiring medical clearance and immunizations.

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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