Research Associate II-Molecular & Cell Biology
Job ID: req2826
Employee Type: exempt full-time
Division: Laboratory Animal Science Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA
The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.
Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.
Laboratory Animal Sciences Program (LASP) provides exceptional quality animal
care and technical support services for animal research performed at the
National Cancer Institute at the Frederick National Laboratory for Cancer
Research. The Center for Advanced Preclinical Research (CAPR) within LASP
develops preclinical model systems for evaluation of cancer therapeutics
and biomarker discovery, facilitating the translation of research findings to
the clinic. Mouse models are developed or retooled for application to studies
assessing small molecule drugs, immunotherapy, and other cutting-edge cancer
treatments. The successful candidate will be engaged in in vitro biomarker
studies evaluating a variety of experimental anticancer agents in tissues
derived from mouse models of cancer, as a member of CAPR’s Preclinical
Evaluation team. The RAII will perform a key role in laboratory studies
supporting translational research, by analyzing the molecular and pathway
effects of experimental therapeutics in mouse models for cancer. The RAII will
also assist in the collection, preparation, analysis and storage of tumor
tissue samples as well as initiate primary tumor cell culture. Duties may
include basic cell and molecular laboratory work such as cell line maintenance
and animal genotyping, and responsibility for lab maintenance and writing SOPs.
He/she will maintain strong working relationships with other team members as
well as with CAPR collaborators.
- Conduct experimental assays on
tumor tissues and cells
- Perform molecular biology
procedures such as nucleic acid extraction and purification, PCR, and Western
- Establish primary tumor cell
lines from mouse and human tumor tissue
- Maintain cancer cell lines in
culture and perform drug sensitivity assays on cancer cell lines
- Viral infection and transient
transfection of cell lines
- May implement and troubleshoot
- Modify and update protocols as
- Execute experiments
independently with guidance from program scientists
- Maintain proper quality control
checks for experiments, assays, and equipment. General laboratory upkeep.
- Compile, analyze, and report
scientific data to program scientists
- Keep accurate and detailed
records and documents laboratory operations and data collection.
- Computer/data management,
including maintenance of complex Excel spreadsheets, GraphPad Prism software.
- Perform assignments independently
and following all applicable regulations, policies, and procedures
- Work involves the use of potentially
hazardous material or may be located in areas where such materials are
- Work may require the use of personal protective equipment and
requires adherence to strict safety protocols.
- Schedule may include occasional
weekend and holiday hours.
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a Bachelor's degree from an accredited
college/university in a field related to biomedical research or four (4) years
equivalent experience in the appropriate field in lieu of degree. Foreign
educated candidates who have completed part or all of their education outside
of the United States must have their foreign education evaluated by a
company-approved accrediting organization to assure that it has met the
equivalency of the qualifications of degree work in the United States.
- In addition to educational
requirements, a minimum of five (5) years job-related biomedical experience.
- Ability to obtain and maintain a security clearance.
Candidates with these desired skills will be given preferential consideration:
- Expertise in molecular
biology procedures such as nucleic acid extraction and purification, PCR, and
- Experience with primary tissue
culture and cell line maintenance.
- Cancer biology experience
preferred but not essential.
- Excellent organizational skills.
- Experience with CRISPR
methodology a plus but not required.
- Experience with flow cytometry a
plus but not required.
- This position is subject to working with or have potential for exposure to animal(s) and/or animal
- This position is subject to working with or have potential for exposure to infectious material, requiring
medical clearance and immunizations.
- This position may require continuous or intermittent exposure to and exertion in environmental
conditions such as working outdoors, temperature extremes, humidity extremes. etc. as part of the job.
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)