Job ID: req2821
Employee Type: nonexempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA
The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.
Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.
The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI’s Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates. The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. A secondary mission is to evaluate the efficacy of approved oncology drugs in combination or as single agents in a subset of rare and recalcitrant PDX tumor models.
- Establish 2D cell cultures from patient samples and from tumors
isolated from mice implanted with human tumors
- Aid in the daily maintenance, passaging and cryopreservation of
the various 2D & 3D cultures grown in different medias
- Perform routine cell culture tasks including cell line
maintenance, preparation of large batches of cells (25-150 flask harvests) for
drug studies or cryopreservation, growth of cells on a variety of supports.
- Prepare all required medias, components and reagents used in the
culture of 2D and 3D cell cultures
- Participate in the maintenance of proper records is critical as
each patient derived cell mixture/clone/line will be passaged and expanded
multiple times with the intent of delivering carefully documented samples to
- Aid in other routine tasks as needed such as cell photography,
tube/plate labeling, cell deliveries/pick-ups etc
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
of a BS/BA degree from an accredited college or university (according to CHEA).
(Additional qualifying experience may be substituted for the required
education). Foreign degrees must be evaluated for U.S. equivalency.
to be flexible to meet changing needs
skills and database management skills
to work in a fast paced environment
to work under Biosafety Level-2 conditions
- Ability to obtain and maintain a security clearance
of Microsoft office programs (Word, Excel, PowerPoint)
skills and database management skills
to maintain precise records
written and verbal communication skills and attention to detail
Candidates with these desired skills will be given preferential consideration:
- Knowledge of sterile eukaryotic tissue culture techniques
- Knowledge of organoid cell culture
- Job hazard or
requirement Enroll in OSHA BBP.
- Must be medically cleared to receive
any necessary immunizations per protocol requirements.
hazard or requirement Employee would receive vaccinations required
and determined by Occupational Health Services (OHS) and/or mandated by Federal
Health Standard Regulations
hazard or requirement
- This position is subject to working with or have potential for exposure to animal(s)
and/or animal material(s)
- This position is subject to working with or have potential for exposure to infectious
material, requiring medical clearance and immunizations
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)