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Research Associate II, Vector Biosciences

Employer
Astellas Pharma US, Inc.
Location
South San Francisco, California
Start date
Aug 4, 2022

View more

Discipline
Regulatory, Science/R&D, Biology, Research
Required Education
Bachelors Degree
Position Type
Full time
Hotbed
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About Astellas Gene Therapies

Astellas Gene Therapies is an Astellas Center of Excellence developing genetic medicines for patients with rare, life-threatening diseases. Based on our innovative approach to developing genetic medicines, as well as our industry-leading internal manufacturing capability and expertise, we have become the Astellas Center of Excellence for their newly-created Genetic Regulation Focus Area. We are currently exploring three gene therapy modalities: gene replacement, exon skipping gene therapy, and vectorized RNA knockdown, with plans to expand our focus and geographic reach under Astellas. We are based in San Francisco, with manufacturing and laboratory facilities in South San Francisco and Sanford, North Carolina. For more information, please visit our website: www.astellasgenetherapies.com

About Astellas

Astellas Pharma Inc. is a pharmaceutical company conducting business in more than 70 countries around the world. We are promoting the Focus Area Approach that is designed to identify opportunities for the continuous creation of new drugs to address diseases with high unmet medical needs by focusing on Biology and Modality. Furthermore, we are also looking beyond our foundational Rx focus to create Rx+® healthcare solutions that combine our expertise and knowledge with cutting-edge technology in different fields of external partners. Through these efforts, Astellas stands on the forefront of healthcare change to turn innovative science into value for patients. For more information, please visit our website at https://www.astellas.com/en.

Our Values:

  • Be BOLD (Find a Way)

  • Care Deeply -- for our patients, each other and our work

  • #GSD (Get Stuff Done)

What is the catalyst for you in your professional career? Are you ready to be a part of a bold, innovative company, to make a change and to make a difference? Listed below is a current opening for a smart, driven, experienced, and just plain nice individual who wants to join us and be a part of our journey.

The Role

The Research Associate will support the Vector Biosciences team and assist with the development, characterization, and qualification of in vitro potency assays for AAV vector products. The Research Associate will be located in our South San Francisco R&D laboratories.

Primary Responsibilities

  • Perform routine cell culture work, including thawing, maintaining, expanding, cryopreserving cell lines and/or primary cells

  • Execute cell-based assays to test AAV vector candidates using routine procedures (AAV transduction, transfection, and cell differentiation)

  • Perform DNA, RNA, and protein isolation from mammalian cells

  • Analyze gene and protein expression through the application of a variety of techniques (RT-PCR, qPCR, digital PCR, western-blot, immunofluorescence, and ELISA)

  • Collect, process, and evaluate data; Accurately document assay results and analyze data through the maintenance of an updated laboratory notebook, prepare study reports, and communicate results and research progress in lab meetings as needed

  • Participate in sample management activities such as receiving, processing, storage, and shipping pre-clinical samples

  • Collaborate with other teams to receive and aliquot vector lots, document characterization of vectors (vg titer, capsid titer, alkaline gel results)

  • Collaborate in laboratory management activities such as receiving, tracking, storage, and maintenance of laboratory reagent stock

  • Additional duties as assigned

About you

Must Have/Required

  • Bachelor's degree in Molecular Biology, Biology, Life Sciences or a related discipline with 2+ years of experience or a master's degree with 1+ years of experience in molecular and cell biology techniques

  • Proven hands-on experience with sterile technique for handling and growing mammalian cell lines, including maintenance, cell characterization and differentiation, expansion, and banking

  • Demonstrated experience with routine molecular biology techniques (DNA/RNA/protein purification, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence)

  • Ability to work both independently and in a team-oriented environment to meet project goals and timelines

  • Strong written and verbal communication skills

  • Models our Core Values: Be Bold, Care Deeply, #GetStuffDone -- is experienced as someone who exemplifies the culture we want to create; operates with transparency; is trusted

May Have/Preferred

  • Experience working with viruses, especially Adeno-Associated Virus (AAV)

  • Experience working with in vitro neuromuscular models

  • Experience with ELISA, luciferase, and/or enzymatic activity assays

  • Experience working in cell-based potency assays

  • Industry experience

Benefits:

  • Medical, Dental and Vision Insurance

  • Generous Paid Time Off options, including Vacation and Sick time, plus national holidays including year-end shut down

  • 401(k) match and annual company contribution

  • Company paid life insurance

  • Annual Corporate Bonus and Quarterly Sales Incentive for eligible positions

  • Long Term Incentive Plan for eligible positions

  • Referral bonus program

Category Vector Biosciences

Astellas is committed to equality of opportunity in all aspects of employment. EOE including Disability/Protected Veterans

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