The Krogan Lab (http://kroganlab.ucsf.edu) is a highly dynamic and collaborative laboratory focusing on the molecular networks underpinning neurological disorders, infectious disease, and carcinogenesis. You will be focusing on culturing iPSCs and iPSC-derived cell types (e.g. neurons, cardiomyocytes) and mapping protein-protein interactions (PPI) and signaling networks in healthy state and disease using mass spectrometry. This role is an excellent opportunity to learn a number of valuable, cutting-edge techniques in proteomics while working with a dynamic and interdisciplinary research team. The candidate will characterize new iPSC lines expressing affinity-tagged proteins of interest for PPI mapping, prepare samples for proteomic analysis and perform functional validation studies involving CRISPR-based technologies and imaging. The candidate will also have the opportunity to develop and implement new protocols for mass spectrometry-based proteomics experiments in neurons and other cell types.
Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently. Candidate’s CV and/or cover letter must state basic qualifications (or if pending) upon submission. Candidates must meet all qualifications by the time of hire.
• Jr. Specialist rank: Bachelor’s degree in biological sciences or an appropriate scientific field.
• Assistant rank: Master’s degree, or Bachelor’s degree and 5-10 years of work experience in biological sciences or an appropriate scientific field.
• Associate rank: Master’s degree, or Bachelor’s degree and 5-10 years of work experience in biological sciences or an appropriate scientific field, in addition to a record of academic accomplishments, including contributions to published or in-press research in the field and a demonstrated record of University and/or public service.
• Experience in Molecular biology techniques including molecular cloning, PCR, and western blotting.
• Cell/tissue culture experience.
• Able to work independently and meet deadlines; recognize and resolve problems while maintaining a high level of organization.
• Effective verbal and written communication skills to interface with the team to review and analyze the results.
• Experience in Microsoft Office (Word, Excel, and/or Powerpoint).
• Experience culturing iPSCs and iPSC-derived cells.
• Experience with neuronal or cardiomyocyte cultures.
• Generating new cell lines and characterizing them.
• with CRISPR/gene editing techniques
• DNA/RNA Blotting.
• Transfection or viral transduction.
• Affinity purifications of proteins.
• Preparation for samples for mass spectrometric analysis.
• Fluorescence microscopy.
• Experience with mass spectrometry data.
• Experience with managing large datasets and/or databases.
• Experience with statistical data analysis
Please apply online at https://apptrkr.com/3227090, with a CV, cover letter, and two reference contacts.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.