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Scientist, TCR & pHLA Engineering & Production

Employer
Alloy Therapeutics
Location
Waltham, MA
Start date
Jul 20, 2022

View more

Discipline
Manufacturing & Production, Science/R&D, Biotechnology
Required Education
Bachelors Degree
Position Type
Full time
Hotbed
Genetown
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Job Details

Scientist, TCR & pHLA Engineering & Production – Waltham, MA

About Alloy Therapeutics

Alloy Therapeutics is a biotechnology ecosystem company empowering the global scientific community to make better medicines together. Through a community of partners, Alloy democratizes access to tools, technologies, services, and company creation capabilities that are foundational for discovering and developing therapeutic biologics. The company facilitates affordable, non-exclusive access to the entire drug discovery community from academic scientists, small and medium biotech, to the largest biopharma. Alloy’s lead offering, the ATX-Gx™ platform, is a human therapeutic antibody discovery platform consisting of a growing suite of proprietary transgenic mice strains. Alloy is a leader in bispecific antibody discovery and engineering services, utilizing its proprietary ATX-CLC common light chain platform integrating novel transgenic mice and phage display. Alloy is headquartered in Boston, MA with labs in Cambridge, UK; Basel, CH; San Francisco, CA; and Athens, GA. As a reflection of Alloy’s relentless commitment to the scientific community, Alloy reinvests 100% of its revenue in innovation and access to innovation.

At Alloy, we believe our industry should compete on getting the best drugs to patients as quickly as possible, not exclusive access to the best platforms. The team and company are rapidly expanding; successful members thrive in our shared culture of accountability, deliberate trust, and open communication. As a team we aspire to work together to exceed expectations and collectively contribute across the global organization to always maintain our nimble, startup culture.

MAY THE BEST DRUG WIN.

 

About Scientist, TCR & pHLA Engineering & Production – Waltham, MA

KeywayTM TCR Discovery group is looking for a highly motivated and enthusiastic Scientist to join our team in Waltham, MA. This position will focus on T cell receptors (TCRs) and peptide Human Leukocyte Antigen complex (pHLA) engineering and production to support the discovery of novel TCR biotherapeutics. The candidate must have in-depth knowledge of current state-of-the-art T cell receptors engineering and production and be able to apply these practices at the bench. This role will drive technology developments and optimization of pHLA complex and soluble TCR production in prokaryotic and eukaryotic systems to improve TCR drug developability for development as a drug candidate. 

Principal Responsibilities

  • Develop prokaryotic and eukaryotic cell-based expression system for pHLA complex and soluble TCR production
  • Lead pHLA and TCR material generation to support discovery campaigns and downstream functional characterization
  • Run bispecific and multispecific functional TCR engineering and production
  • Perform E. coli protein inclusion body extraction, purification, and refolding
  • Perform protein purifications and troubleshoot any issues with purification using AKTA systems
  • Use automation to support soluble TCR reformatting, production, purification, and screening
  • Express and produce selected TCR leads in micro-scale in a high-throughput manner
  • Characterize TCR binder properties in various affinity and activity assays, like ELISA, iQue, FACS, Octet, epitope binning, reporter gene assays
  • Engineer and improve binders by means of affinity maturation
  • Excellent written and oral communication skills are necessary in order to succeed in a cross-functional scientific environment
  • Multitask and accomplish goals under project and team timelines
  • Be generous when others are needy, greedy, or fearful

 

Required Qualifications and Skills

  • PhD in molecular biology, biochemistry, cell biology, or related field; MS with 4+ years of industry experience; or BS with 6+ years of industry experience; Title will be aligned with experience level
  • Deep understanding of protein chemistry, including analyses, structure, and function
  • Experience purifying and characterizing proteins, ideally antibodies and antibody fragments
  • Experience with biophysical tools: DSC, DSF, DSC, ce-SDS, cIEF, mass spectrometry
  • Proven track record in protein expression, purification, and engineering
  • Skilled at FACS sorting and flow cytometric analysis
  • Experience with phage display, yeast display, mammalian library, and screening are a plus
  • Structure-guided protein engineering is preferred
  • Ability to conduct data analysis, interpret experimental results, and independently draw conclusions

 

Benefits Include 

  • Competitive base salary based on the position and your experience level
  • Significant opportunities for advancement inside Alloy, through our affiliated venture studio, 82VS, and across our partner companies
  • Equity compensation in a company with incredible growth potential and a 30+ year plan
  • Generous personal and family medical, dental and vision benefits with 90% of premiums and deductibles covered by Alloy, company-paid disability insurance, and an annual stipend for continuing education
  • Flexible schedule, unlimited vacation as long as the job gets done, medical leave including time caring for sick family members, and parental leave all built around our team-driven philosophy
  • Commitment to your career through individualized professional development plan for all employees to ensure good role and skill fit either at Alloy or your next biotech company
  • Additional benefits including adoption support, IVF/PGT support, compensated time for bone marrow donation, continuing education, leadership development, and others

Company

Alloy Therapeutics is a biotechnology ecosystem company empowering the global scientific community to make better medicines together. Through a community of partners, we democratize access to tools, technologies, services, and company creation capabilities that are foundational for discovering and developing therapeutic biologics. We provide affordable, non-exclusive access to the entire drug discovery community from academic scientists, small and medium biotech, to the largest biopharma.

Our lead offering, the ATX-Gx™ platform, is a human therapeutic antibody discovery platform consisting of a growing suite of proprietary transgenic mice strains.

Founded in 2017 and privately funded by visionary investors, we are headquartered in Boston, MA with European labs in Cambridge, UK.

As a reflection of our relentless commitment to the scientific community, Alloy reinvests 100% of its revenue in innovation and access to innovation.

Find Us
Website
Location
44 Hartwell Ave
Lexington
MA
02421
United States
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