Scientist - Enzymology of Protein and RNA Degradation

Thousand Oaks, CA
Jun 09, 2022
Required Education
Masters Degree/MBA
Position Type
Full time

Job Description


Youve earned your degree. How will you use that achievement to reach your goals? Do more with the knowledge youve worked hard to acquire and the passion you already have. At Amgen, our shared missionto serve patientsdrives all that we do. It is key to our becoming one of the worlds leading biotechnology companies, reaching over 10 million patients worldwide. Become the professional you are meant to be in this meaningful role.

Scientist - Enzymology of Protein and RNA Degradation


What you will do

Lets do this. Lets change the world. In this vital role you will be a part of our Biochemistry and Structural Biology (BSB) group. We are seeking an accomplished Scientist to contribute to the Induced-Proximity Platform (IPP) group tasked with the discovery of novel therapeutics in the Targeted Protein and RNA Degradation fields.

The BSB group is focused on enhancing our understanding of the biochemistry of ubiquitin ligases and other related enzymes involved in cellular protein and RNA degradation. These efforts including the discovery of Proteolysis and Ribonuclease-targeting Targeting Chimeras (PROTACs and PRIBOTACs) and Molecular Glues to advance Amgens drug discovery pipeline of intractable and difficult-to-drug therapeutic targets.

This is a unique opportunity for those who are interested in conducting basic research in the targeted macromolecule degradation field while directly impacting therapeutic discovery programs at a premier organization.

The candidate will work collaboratively with researchers across multiple functions within the IPP department as well as across disease therapeutic areas and the Therapeutics Discovery organization. The successful candidate will also work with external partners and contract research organizations (CROs) and have the opportunity to present scientific work at national and international conferences.

Key Responsibilities
  • Planning, design, implementation, and documentation of laboratory studies related to the studying of protein complexes involved in protein degradation.
  • Design of DNA constructs and the use of rational and computational tools for the engineering of protein constructs to ensure successful protein expression, purification and structural determination.
  • Perform expression, purification, and characterization of recombinant proteins to support various aspects of drug discovery efforts related to Targeted Protein and RNA Degradation.
  • Perform biochemical, biophysical and structural characterization of protein complexes.
  • Study of protein-protein, protein-RNA interactions and enzymology of protein ubiquitination reactions.
  • Collaborate within and across interdisciplinary teams to advance our understanding of Targeted Protein and RNA Degradation mechanisms.

What we expect of you

We are all different, yet we all use our unique contributions to serve patients. The qualified professional we seek is a Scientist with these qualifications.

Basic Qualifications
  • PhD in Biochemistry, Protein Chemistry, Structural Biology, Chemistry or Chemical Engineering.
  • Strong scientific reasoning, data analysis and problem-solving skills as evidenced by high impact scientific publications.
  • Highly motivated to work in a cross-functional and fast-paced environment.
  • Work independently to plan, lead, and perform experiments.
  • Ability to develop a deep understanding of projects aimed at discovering novel therapeutics.
  • Strong understanding of basic protein biochemistry and biophysics principles, including those pertaining to enzyme kinetics, protein complex formation, and protein-substrate interactions.
Preferred Qualifications
  • 2+ years of postdoctoral or industry experience.
  • Prior experience with the purification and the biochemical and/or biophysical characterization of challenging proteins including protein and RNA complexes.
  • Expert user of FPLC (AKTA PURE or Avant) and HPLC systems (Waters or Agilent).
  • Solid understanding of protein analytical techniques to study macromolecules including SEC, SEC-MALS, DLS, thermal shift assay, mass photometry and LC-MS.
  • Experience in protein expression in E.coli and insect cells.
  • Familiarity with protein modeling and design tools such as MOE, Rosetta or Schrdinger.
  • Knowledge of protein/RNA degradation mechanisms and in particular the biochemistry of ubiquitin ligases or RNases.
  • Practical knowledge of techniques to investigate protein interactions such as HDX, XL-MS, SPR and SAXS is a plus.
  • Practical experience in structural biology including crystallography or cryoEM as demonstrated by solving molecular structures of protein complexes is a desirable but not a requirement.

Join Us

If you're seeking a career where you can truly make a difference in the lives of others, a career where you can work at the absolute forefront of biotechnology with the top minds in the field, you'll find it at Amgen.

Amgen, a biotechnology pioneer, discovers, develops and delivers innovative human therapeutics. Our medicines have helped millions of patients in the fight against cancer, kidney disease, rheumatoid arthritis and other serious illnesses.

As an organization dedicated to improving the quality of life for people around the world, Amgen fosters an inclusive environment of diverse, ethical, committed and highly accomplished people who respect each other but compete intensely to win. Together, we live the Amgen values as we continue advancing science to serve patients.

Amgen is an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status.

We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation.

Amgen requires all staff in the United States, Puerto Rico and Canada to be vaccinated from COVID 19 as a condition of employment. In accordance with applicable law, Amgen will provide reasonable accommodations to staff members who qualify on the basis of a medical reason or a sincerely held religious belief, practice, or observance. Such accommodation may not pose an undue hardship to Amgen, its operations, or its staff.