Scientist, Antibody Discovery – Transgenic Platform
The Scientist, Antibody Discovery is responsible for cell and molecular biology to develop and support the transgenic antibody platform.
- Working with a small but focused team, the Scientist will play an important role in advancing the client’s antibody discovery platform. The candidate will work in a collaborative environment, both within the team as well as externally with CRO service providers.
- The deliverables for this team are focused on using gene editing strategies together with transgene design and delivery to engineer mouse ES cells. The engineered ES cells are in turn used to make transgenic mice to support the platform needs.
- Immunologic characterization of transgenic mice and cells. To include ELISA, FACS/ flow analysis, and specific immune assays related primarily to B cell function and immunoglobulin expression, immune response, affinity, and Ab diversity.
- Specific molecular characterization of immune responsiveness, including Next-gen sequencing and Ab diversity analysis, epitope binning, immunization, and principles of vaccine technologies.
- PhD degree plus 2-5 years experience, or Masters degree plus 3-5 years relevant experience in transgenic and immunologic technologies. The candidate will have patents or peer reviewed publications around relevant topics, and a record of innovation and adaptability.
- Must be able to devise strategies and apply these at the bench for diverse transgenic engineering projects – these include novel antibody gene donors as well as specific therapeutic targets. Knowledge of gene delivery, integration, and genomic analysis required
- Must have demonstrated experience and skill in ES cell culture and transfection, pluripotency assays, large DNA construct generation and handling, quantitative DNA/RNA genotyping and analysis, some in vivo mouse or rodent work, prep work for NGS analysis, and facility with the software programs to perform these analyses
- Must be proficient at record-keeping and staying current with recording the work performed (e.g., in ELN and other database formats). Candidate will be expected to quantify and summarize results of his/her experiments, and present these to the team and/or upper management.
- Familiarity with all aspects of the transgenic process, from transgene design to construction, generation and characterization of cells and transgenic mice, and breeding and phenotyping of the resultant offspring.
- Experience in standard molecular cloning, vector design and transfection technologies, gene expression assays, and if possible, immunological testing techniques such as ELISA, FACS, and/or homogeneous assays
- Ability to design experiments from beginning to end, involving fit for purpose strategies.
- Ability to work as part of a high performing and integrated team, able to respond and adapt to changing timelines; strong organizational skills, including familiarity with MS Office and associated scientific applications to record work, and to manage, analyze and interpret data (including statistical analysis).
- Strong presentation skills to share findings with peers and Sr. Management.