Senior Research Associate/Associate Scientist/Senior Associate Scientist, Technology Development -

Location
Boston, MA
Posted
May 04, 2022
Ref
5601068002
Required Education
Bachelors Degree
Position Type
Full time

Shape Therapeutics is a biotechnology company developing breakthrough gene therapy technologies to treat the world’s most challenging diseases. ShapeTX gene therapy platform comprises RNAskip™, RNAfix™, and RNAswitch™ payload technologies, next-generation tissue-specific AAVid™ delivery technology, and SquareBio, a solution to scalable gene therapy manufacturing based on industrialization of human stable cell lines. At the core of these technologies reside ShapeTX AI analytic platform, where data drives decisions in building technology today to enable gene therapies of tomorrow. ShapeTX is committed to data-driven scientific advancement, passionate people, and a mission of providing life-long cures to patients. Shape Life!

At ShapeTX, we are a dynamic team of professionals who are dedicated and passionate about making cures a reality. Through diversity of thought, scientific knowledge, professional rigor and focus we are merging cutting-edge science with extensive drug development expertise to unlock cures to many debilitating diseases.

Shape Therapeutics is headquartered in Seattle, Washington with a satellite site in Boston, Massachusetts.

ShapeTX is looking for a Senior Research Associate / Associate Scientist / Senior Associate Scientist with a background in molecular biology and mammalian cell culture to join our Technology Development team in Boston, MA. In this position, the successful candidate will advance technology development across ShapeTX's novel gene therapy platforms, in particular (but not limited to) RNA editing. This role would use a design-build-test synthetic biology approach along with high-throughput, multiplexed assays to improve gene therapy constructs.  At a minimum, the candidate must have experience in plasmid cloning and culturing cell lines. The ideal candidate will have experience in next-generation sequencing, cell line engineering, and single-cell analysis. Attention to detail and interest in learning new cutting-edge techniques is essential.

ShapeTX has developed a suite of technology platforms that broadly enable RNA targeting, RNA editing and RNA replacement for diseases with high unmet need. By leveraging enzymes already present in human cells such as ADAR (adenosine deaminase acting on RNA), RNA editing eliminates the immunogenicity and malignancy risks associated with DNA-based editing platforms such as CRISPR. RNA editing requires no foreign proteins, just the delivery of a single guide RNA that can be gene-encoded and packaged in AAV for precise delivery. RNA editing can be fine-tuned to achieve the desired biological outcome and, together with our AAVidTM capsid discovery platform and SquareBio stable cell line manufacturing platform, we can target diverse tissues and overcome current industry manufacturing challenges. Come join the team working to harness the power of programmable RNA medicines to improve patients’ lives!

Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs February 2022. Katrekar, et al. Nature Biotechnology

Roche's gene therapy plans take Shape with $3B-plus biobucks deal August 2021. Fierce Biotech

Shape Therapeutics Secures $112 Million in Series B Financing to Advance Breakthrough RNA Technology Platforms to Democratize Gene Therapy July 2021. Globe Newswire

Roles and Responsibilities

  • Execute high throughput screening methods in cultured cell lines to evaluate guide RNAs for therapeutic RNA editing, including: gene synthesis; cloning plasmid libraries; mammalian cell culture and transfection; RNA purification; amplification of target genes by qPCR and droplet digital PCR; Next-Generation Sequencing; single-cell barcoding
  • Design and plan well-controlled experiments. Seek opportunities to improve our current protocols and workflows. Troubleshoot as needed to find creative solutions.
  • Assist with lab operations as needed (setting up new lab space, consumables ordering, equipment maintenance) and generally being a good lab citizen.
  • Support and collaborate on multiple disease projects in a fast-paced startup environment, working across teams at ShapeTx.
  • Interpret and communicate results to team members to drive key decisions and future experiments.

Qualifications and Requirements

  • Bachelor's or Master's degree in a related discipline, with at least 2 years working in a laboratory setting.
  • Attention to detail, diligent record-keeping, organization, and time management.
  • An interest in technology development, learning new techniques, and inventing novel methods.

Skills and Experience

  • Experience with molecular biology techniques: RNA / DNA purification and sequencing; plasmid design and cloning; PCR.
  • Preferred experience in reverse transcription, library cloning, and Next Generation Sequencing.
  • Experience with mammalian cell culture and transfection, particularly cell line engineering using Crispr/Cas9, integrases, transposons, or viral transduction.

 

We aspire to Shape Life! not only through our science, but also through our commitment to foster an inclusive environment that supports diverse perspectives and experiences.