Laboratory Associate - Protein Production

Brisbane, CA
Apr 29, 2022
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Mammoth is seeking a Laboratory Associate with protein production expertise to join its growing Discovery team. The Discovery team is responsible for developing novel CRISPR-associated (Cas) nucleases for genome editing applications. 
The successful candidate will be responsible for executing laboratory workflows to produce Cas nucleases for genome editing. The primary focus for this role will be on protein production and process development. This is an excellent development opportunity for researchers who want to learn new skills and start their career working on cutting-edge CRISPR technologies.
  • Execute internal microbial protein production for research applications
  • Contribute to process development to establish methods to produce proteins and protein complexes for biochemical characterization and structural interrogation
  • Rigorously document newly developed methods and results
  • Provide regular updates at internal meetings
  • Other duties as assigned

  • Bachelor’s degree in molecular biology, biochemistry, or a related field
  • 2+ years of laboratory experience in academia or industry developing and executing bacterial protein expression workflows including, but not limited to:
  • Bacterial transformation and growth in liquid media
  • Execution of upstream (cell harvest, lysis, clarification) and downstream (liquid chromatography, filtration, concentration) processes
  • Protein analytical methods (Eg. homogeneity evaluation, protein quantitation, detection / quantitation of process-related impurities such as host-cell protein, endotoxin, nucleic acids)
  • Generation of working cell banks
  • FPLC systems (Eg. Cytiva AKTA, BioRad NGC) and associated software packages

  • Prior experience executing upstream and / or downstream process development
  • Previous protein manufacturing experience (research-grade or cGMP)
  • Hands-on experience in the following areas: 
  • Biochemical assays
  • Molecular biology (PCR, DNA/RNA purification and quantitation)
  • Computer and scripting skills (Benchling, Geneious, JMP, SQL, R, python)

  • Company-paid health/vision/dental benefits
  • Unlimited vacation and generous sick time
  • Company-sponsored meals and snacks
  • Wellness, caregiver and ergonomics benefits
  • 401(k) with company matching

Mammoth Biosciences is harnessing the diversity of life to power the next generation of CRISPR products. Through the discovery and engineering of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. Mammoth aims to develop permanent genetic cures through best-in-class in vivo and ex vivo therapies and to democratize disease detection with on-demand diagnostics. Mammoth can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Based in the San Francisco Bay Area, Mammoth Biosciences is co-founded by CRISPR pioneer and Nobel Laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Redmile Group, Foresite Capital, Senator Investment Group, Sixth Street, Decheng, Mayfield, NFX, and 8VC, along with leading individual investors including Brook Byers, Tim Cook, and Jeff Huber.

Our small-size proteins, such as Cas14 and CasPhi, are poised to transform disease treatment enabling novel editing approaches and delivery options that are difficult to achieve or not even possible with previously described CRISPR-Cas systems. Our teams are responsible for discovering and engineering novel CRISPR systems, establishing CRISPR-based diagnostics, and translating our unique CRISPR technology into therapeutic treatments with the ultimate goal of cures for indications with high medical need.

It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law.  This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation.