Senior Scientist, Cell Engineering

Senior Scientist, Cell Engineering

The Position:

Bolt Biotherapeutics is seeking a talented Senior Scientist to join the Department of Immunology & Pharmacology. The candidate will report to the Director of Molecular and Cellular Biology and will develop cell lines and assays to support screening and pre-clinical development for the pipeline including Bolt’s novel Boltbody™ Immune-stimulating Antibody Conjugates (ISACs) drug candidates. Bolt promotes a highly interactive intellectual environment. The ideal candidate will be experienced in vector design and generation of modified cell lines. A strong background in cancer biology, immunology or cell biology is a plus.

Key Responsibilities:

• Lead cell line development for all of research
• Coordinate cell line needs across research by leading regular meetings with stakeholders
• Modify cell lines by introduction of exogenous genes, knockout of endogenous genes, or knockdown of expression
• Design Plasmid and lentiviral constructs
• Design CRISPR guide RNAs for gene knockout, etc.
• Generate pools and clones by cell sorting
• Develop reporter and other cell based assays

Job Requirements:

• PhD in Cell Biology, Immunology, or related field with 5-10 years of experience in biologic or cellular therapeutic discovery / development.
• Experience using various protein expression vectors, construct design, cloning, PCR, primer design, use of CRISPR technology.
• Cell transduction/transfection, electroporation, flow cytometry / soring, immunofluorescence microscopy, reporter assays, ELISA, SDS-PAGE/western blotting.
• Strong presentation and written communication skills.
• Strong leadership and collaborative skills.

Who We Are:

Bolt Biotherapeutics, based in the San Francisco Bay Area, is a clinical-stage biotechnology company developing Boltbody™ ISACs, a new class of immuno-oncology therapeutics that have eliminated tumors following systemic administration in preclinical studies while also stimulating immunological memory, which may lead to more durable clinical responses for patients. This is a unique opportunity to join and build, with likeminded colleagues, a company that will transform the lives of individuals with cancers.

We are an Equal Opportunity Employer offering a competitive salary and benefits package.