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Scientist

Employer
Medical Science & Computing (MSC), a Dovel company
Location
Bethesda, Maryland
Start date
Jan 16, 2022

View more

Discipline
Science/R&D, Biotechnology
Required Education
Bachelors Degree
Position Type
Full time
Hotbed
BioCapital

We are currently searching for a Scientist to Investigate the pathways of genome metabolism involving helicase enzymes defective in hereditary disorders displaying age-associated clinical features and/or cancer. Understand the molecular and cellular roles of human DNA helicases in pathways that are important for the maintenance of genome integrity and cellular homeostasis. Genetic and biochemical approaches are used to characterize the roles of helicases in cellular nucleic acid metabolism through their catalytic functions and protein interactions. Use of siRNA/CRISPR screens, pharmacologically active small molecules, single-molecule DNA fiber studies, live cell imaging by immunofluorescence confocal microscopy, protein biochemistry, and enzyme kinetic assays. 

 

This opportunity is full-time, and it is on site in Bethesda, MD.  

 

 

Duties & Responsibilities
  • Perform investigations with reagents material/ tissues under investigation which range from cellular products to whole tissue; maintain inventory system and sample storage; maintain inventory of all experiments.
  • Perform biochemical assays including electrophoretic mobility shift assay EMSA, helicase assays with radiometric or fluorometric substrates, ATPase assays, protein interaction assays ELISA, co-immunoprecipitation, dot blot, affinity pull-down.
  • Present ongoing research and findings at weekly lab unit section meetings.
  • Perform mathematical calculations for the preparation of graphs.
  • Write and edit scientific papers for submission to peer-reviewed journals.
  • Present papers/abstracts/posters at scientific meetings/conferences.
  • Design and synthesize nucleic acid substrates for biochemical assays to analyze proteins involved in nucleic acid metabolism.
  • Work with staff on the maintenance of animal protocols; work with animal facility staff to maintain, design and produce the necessary crosses of distinct mouse strains Safeguard animal logs; work with animal facility staff to maintain and breed specific mouse models.
  • Work with staff on the maintenance of laboratory protocols; work with facility staff to maintain, design and produce the necessary safety protocols and procedures.
  • Perform molecular biology experimentations, bioinformatics, gene and protein profiling, protein concentrations in extracts.
  • Express recombinant proteins using a baculovirus system and purify recombinant proteins using both conventional biochemical and chromatography steps, as well as design protocols and operate FPLC instrumentation.
  • Work with staff on scheduling and maintenance of research tracking system and databases for all research activities, daily records of experiments and results, including research documentation.
  • Maintain an inventory system and sample storage per outlined guidelines by making la-bels, tubes, aliquoting and re-inventory of processed samples; ensure that policies and procedures regarding retrieval are followed Maintain an inventory and notebooks of all experiments.
  • Create and maintain a research tracking system and database for all research activities, daily records of experiments and results, including research documentation.
  • Keep up to date on technical developments, especially in the fields of genomic stability, DNA repair, DNA damage response, cellular senescence, nucleic acid metabolism, nucleic acid processing enzymes and binding proteins, recombinant protein expression and purification, single-molecule studies, and single-cell experimentation.
  • Operate laboratory equipment to analyze samples.
  • Use or operate a variety of routine and specialized laboratory instruments and equipment for the collection of experimental data from various molecular, cellular, tissue, and organismal assessments, and analysis of data.

#SOAR

 

#LI-JR1

 

 

Requirements

 

Qualifications:

 

  • Minimum Seven (7) years of experience in Molecular Biology, Protein Biochemistry, or a related field.
  • PhD degree in Biological Sciences or other related discipline.
    • OR Master’s degree plus three (3) years of specialized
    • OR BA/BS degree plus five (5) years of specializedexperience

 

Skills:

  • Knowledge of proper laboratory disposal methods and safety procedures.
  • Ability to work independently and think critically with skilled hands and sound 
  • Strong communications skills, both oral and written.
  • Excellent analytical, organizational and time management skills.
  • Experience with isolation of DNA and RNA from tissues and cells, small samples, PCR, immunohistochemistry, Western blotting, current methodologies of gene targeting and analysis; RNA interference, transfections, and prior experience working with cell models.
  • Familiarity with flow cytometric techniques.
  • Familiarity with tissue culture techniques.
  • Strong familiarity with cell biology, genetics, state-of-the-art molecular biology, and its techniques.
  • In-depth knowledge of single-cell DNA replication and DNA repair assays and single-molecule (kinetic) biochemical assays.
  • Experienced with MS Office.

 

 

 

Due to our contractual requirements and federal orders, including an Executive Order from the White House and an emergency regulation from the Centers for Medicare & Medicaid Services (CMS), the position for which you are applying requires that you provide proof of your vaccination status. If you are unable to receive the COVID-19 vaccine for medical reasons or because of a sincerely held religious belief, you may request an exemption from the vaccination requirement which shall be reviewed after the submission of requested documentation. If an accommodation is granted, the conditions may include weekly testing and masking. All Guidehouse employees also agree to follow any additional health and safety mitigation policies that may be required in the workplace.

 

Company Description

Dovel Technologies and its Family of Companies (Medical Science & Computing and Ace Info Solutions) was acquired in October 2021.

 

Guidehouse is a leading global provider of consulting services to the public sector and commercial markets, with broad capabilities in management, technology, and risk consulting. By combining our public and private sector expertise, we help clients address their most complex challenges and navigate significant regulatory pressures focusing on transformational change, business resiliency, and technology-driven innovation. Across a range of advisory, consulting, outsourcing, and digital services, we create scalable, innovative solutions that help our clients outwit complexity and position them for future growth and success. The company has more than 12,000 professionals in over 50 locations globally. Guidehouse is a Veritas Capital portfolio company, led by seasoned professionals with proven and diverse expertise in traditional and emerging technologies, markets, and agenda-setting issues driving national and global economies.

 

Guidehouse is an Equal Employment Opportunity / Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, ancestry, citizenship status, military status, protected veteran status, religion, creed, physical or mental disability, medical condition, marital status, sex, sexual orientation, gender, gender identity or expression, age, genetic information, or any other basis protected by law, ordinance, or regulation.

 

Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance, including the Fair Chance Ordinance of Los Angeles and San Francisco.

 

If you have visited our website for information about employment opportunities or to apply for a position, and you require accommodation, please contact Guidehouse Recruiting at 1-571-633-1711 or via email at RecruitingAccommodation@guidehouse.com. All information you provide will be kept confidential and will be used only to the extent required to provide needed reasonable accommodation.

 

Guidehouse does not accept unsolicited resumes through or from search firms or staffing agencies. All unsolicited resumes will be considered the property of Guidehouse, and Guidehouse will not be obligated to pay a placement fee.

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