Research Technician
- Employer
- Frederick National Laboratory for Cancer Research
- Location
- Frederick, MD
- Start date
- Dec 1, 2021
View more
- Discipline
- Regulatory, Research/Documentation, Science/R&D, Research
- Required Education
- Bachelors Degree
- Position Type
- Full time
- Hotbed
- BioCapital
Job Details
PROGRAM DESCRIPTION The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI's Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates. The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. A secondary mission is to evaluate the efficacy of approved oncology drugs in combination or as single agents in a subset of rare and recalcitrant PDX tumor models.
KEY ROLES/RESPONSIBILITIES
- Aid in the daily maintenance, passaging and cryopreservation of the various 2D & 3D cultures grown in different medias
- Establish organoid cultures from patient samples and from tumors isolated from mice implanted with human tumors
- Prepare cultures for the various validation assays (flow cytometry, cell sorting, Q RT PCR, soft agar clonal assays, RNA/DNA extraction, the STR identity assay, proliferation assays, etc)
- Prepare all required medias, components and reagents used in the culture of organoid cultures
- Participate in evaluating the effects of different basement membrane extracts, media additives, and different methodology used to establish and grow difficult 2D and 3D cell lines
- Aid in other routine tasks as needed such as cell photography, PowerPoint presentations, tube/plate labeling, cell deliveries/pick-ups etc.
- Maintain precise documentation of each 2D/3D cell line in Excel spreadsheets
BASIC QUALIFICATIONS
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
PREFERRED QUALIFICATIONS
Candidates with these desired skills will be given preferential consideration:
- Knowledge of sterile eukaryotic tissue culture techniques
- Knowledge of organoid cell culture
JOB HAZARDS
- This position is subject to working with or have potential for exposure to animal(s) and/or animal material(s)
- This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations
Company
A rewarding career with global impact
Whether you’re an expert in your field or just starting out, we have a career opportunity for you. We’re always looking for people to join us in fulfilling the mission of the Frederick National Laboratory: discovery, innovation, and success in the biomedical sciences.
Our team of 2,400+ scientists, technicians, administrators, and support staff work at the forefront of basic, translational, and preclinical science, with a focus on cancer, AIDS, and other infectious diseases.
We collaborate with colleagues across the National Cancer Institute, National Institutes of Allergy and Infectious Diseases, and others throughout the National Institutes of Health. We also engage with extramural investigators in academia, government and industry.
Your path to joining our team begins with the desire to work for the only national laboratory dedicated to biomedical research. Our employees share a common desire to help make a difference in cancer research and public health concerns. As you search for a career that fits your education, skills, and abilities, explore the core values that guide us and emphasize work-life balance.
Discover why joining the Frederick National Laboratory team could be the most important career step you take
- Website
- https://frederick.cancer.gov/
- Phone
- 301-846-1000
- Location
-
8560 Progress Drive
Frederick
MD
21701
US
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