Scientist I, Upstream Fermentation

Employer
Sanofi US
Location
92101, San Diego
Posted
Oct 16, 2021
Ref
R2612037
Required Education
Bachelors Degree
Position Type
Full time

As a member of the scientific research team, the Senior Associate Scientist / Scientist I in Upstream  has a hands-on role executing lab scale bioreactors, collecting and processing samples, preparing fermentation experiment documents, and participating in data analysis and process development/improvement discussions. The  Senior Associate Scientist / Scientist I  provides principal scientist / senior management with technical proficiency, scientific creativity and professional concepts and procedures to solve methodical problem.

Key Responsibilities:

Essential duties and responsibilities include the following.  Other duties may be assigned.

  • Carry out experiments and collect, process data under supervision of the principal scientist.

  • Understanding underlying science and fundamental concepts behinds the products in development.

  • Experimentally determine upstream conditions for microbial expression and specific to Synthorx platform.

  • Bioreactor setup, maintenance, and operation.

  • Sampling and sample analysis.

  • Mechanical and instrumentation troubleshooting.

  • Training of junior members.

  • Experimentally determine fermentation strategies to optimize yields for protein expression.

  • Contribute to fermentation process improvement activities using a data driven approach.

  • Collaborate cross-functionally with Platform and Target Development teams to ensure smooth continuity between fermentation and upstream and downstream technology processes.

  • Develop standard operating procedures, document protocols and technology transfer.

  • Prepare and present project updates to ensure timely delivery.

  • Maintain a high standard for quality control including up-to-date data records for all protein deliveries, including basic understanding of mass spectrometry methods for QC.

  • Troubleshoot challenging technical, experimental, and instrumental issues.

  • Consummate ability to work in a highly collaborative, fast-paced, cross-functional environment, with superior oral and written communication skills.

Basic Qualifications:

  • BS in biological science, chemical or biochemical engineering and 5+ years of lab experience or related field; OR MS and 2+ years experience  in  chemical or biochemical engineering of  bio-related fields.

  • Experience with E. coli-based protein expression is highly desirable. The knowledge and experience of eucaryotic expression (yeast, mammalian cell) is plus

  • Experience setting up and operating lab scale bioreactors (DasGIP, BioFlo, AMBER) is a highly desirable. Experience with multiple fermentation mode is plus.

  • Track record of running microbial fermentation processes in fermenter.

  • A strong understanding of cellular physiology to troubleshoot and improve existing cultivation models.

  • Experience developing methods for protein purification.

  • Familiarity with protocols involving extraction from inclusion bodies is a plus.

  • Experience in protein characterization (SDS PAGE, Western Blotting, quantification). Understanding of MS methods for QC is a plus.

  • Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines.

ADA Notations:

  • Frequent communication (talking/hearing).

  • Regular sitting and reaching with arms.

  • Vision requirements: close, distance, peripheral, color and depth perception, and ability to adjust focus.

  • Lifting up to 50 lbs.

  • Computer use: 30% of the time.

  • Noise conditions: Normal office / lab environment.

  • May work near moving mechanical parts or in a  laboratory setting with chemicals, unpleasant odors, and in areas requiring personal protective equipment.

  • Interaction with laboratory equipment, samples, supplies, and laboratory personnel may be required whereby appropriate precautions are to be taken per the Company’s Safety and Injury, Illness and Prevention Plans.

  • Travel: Occasional.

Sanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law.

 

At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.