Research Associate II/III (CONTRACTOR)

Manager: Scientist III
Department: Research
Location: Richmond, CA

Sangamo Therapeutics is a genomic medicine company building value with cutting-edge work across four distinct but complementary technology platforms – gene therapy, cell therapy, in vivo genome editing, and in vivo genome regulation. Using its deep scientific expertise and proprietary zinc finger genome engineering technology, Sangamo is working to create genomic cures for patients suffering from severe genetic diseases for which today’s medicines can only treat symptoms. At Sangamo, we are passionate about transforming lives by building a robust, sustainable, differentiated, innovative, and high value genomic medicine pipeline that addresses patients with life-limiting conditions. Sangamo takes pride in being the first to edit endogenous human genes, first to treat patients with gene edited T cells, and first to treat patients with in vivo genome editing. Our top priority is to meet the needs of patients with commitment and compassion. Along with our commitment to science and to our patients, we also endeavor to create an inclusive environment that promotes and values diversity. Sangamo is a company where individuals can flourish, grow and develop their expertise.

We are seeking top talent to join our mission. Let’s build a better future together.

JOB SUMMARY:  
Sangamo Therapeutics is seeking a Temporary, Research Associate to join our Cell Therapy group, focusing on development of the next generation of cellular therapies. The successful candidate will contribute to the advancement of our CAR Treg platform. The environment is teamwork-oriented, exciting, fast-paced, and offers the opportunity to directly impact the clinical translation of genome editing.

ESSENTIAL FUNCTIONS: 

• Isolation of primary human T (and ideally Treg) cells and their maintenance in tissue culture. Perform lentiviral transduction or in vitro electroporation to achieve gene editing and CAR expression.
• Perform multiparameter flow cytometry analysis of Zinc Finger Nuclease-edited cells and/or CAR-expressing cells. Develop robust new panels for phenotyping and other flow-based immunoassays.
• Perform functional analysis of edited Tregs, including suppression assays, activation, and reporter assays. Cell sorting experience is a plus.
• Perform gene expression analyses by RT-PCR or related methods.
• Clone new constructs and produce lentiviral vectors, including production of lentiviruses and titration.
• Support lab management and infrastructure.
• Document methods, maintain laboratory notebooks and databases.
• Make detailed observations, summarize data, present progress to project team and/or other groups
• Maintain high level of professional expertise through familiarity with scientific literature.  Investigate, create, and develop new methods and technologies to address research challenges for project advancement

EDUCATION, EXPERIENCE AND SKILLS REQUIREMENTS:

• B.Sc. or M.Sc. in a scientific discipline (preferably immunology or molecular and cell biology). 2+ years of experience. Level will be dependent upon education, skills and experience.
• Highly proficient with aseptic cell culture techniques and cell-based assays using primary human cells. Experience in Treg cell isolation and functional assessment a plus.
• Proficient in multiparameter flow cytometry and experienced in applying rigorous, standardized methods to generate high quality data using FlowJo.
• Proficient in molecular methods such as cloning, nucleic acid extraction, PCR. Illumina sequencing experience is a plus.
• Proficient in the use of computer programs for sequencing and DNA construct generation, data analysis and assembly (e.g. Geneious or similar, MS Excel, MS Powerpoint).

OTHER QUALIFICATIONS:

• Excellent verbal and written communication skills.
• Great attention to detail and excellent organization skills.
• Must have a goal-oriented mindset, be capable of prioritizing and working on multiple projects within timelines.
• Ability to work well in a cross functional team environment.

Sangamo is an equal opportunity employer
The above reflects management’s definition of essential functions for this position but does not restrict the tasks that may be assigned. The above duties are representative only; management may assign or reassign duties and responsibilities to this position at any time.

COVID-19

Sangamo requires all U.S. workers to be fully vaccinated against COVID-19 as of their start date.  This requirement is a condition of employment at Sangamo, and it applies regardless of location or classification of role.  If you are unable to receive the vaccine, you will have an opportunity to request a reasonable accommodation consistent with applicable laws.

Note to External Recruiters: Please understand that we do not accept unsolicited resumes from any source other than from the candidates themselves. Any agency or independent recruiter must have a signed copy of our agency agreement before presenting candidates. Submission of unsolicited resumes without our signed agreement will not create any implied obligation on our part.

If you wish to be considered for the presentation of candidates, please contact us through Recruiting@sangamo.com. Attach your contact information, your area of specialty, and any marketing information you wish to provide. Someone form the Talent Acquisition Department will contact you directly if there is a business need for your services.

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