Scientific Associate II (Serology)

Employer
CSL Behring
Location
Cambridge, Massachusetts
Posted
Oct 03, 2021
Ref
R-144091
Required Education
Bachelors Degree
Position Type
Full time

With operations in 35+ nations and ~27,000 employees worldwide, CSL is driven to develop and deliver a broad range of lifesaving therapies to treat disorders such as hemophilia and primary immune deficiencies, and vaccines to prevent influenza. Our therapies are also used in cardiac surgery, organ transplantation and burn treatment.

CSL is the parent company of CSL Behring and Seqirus. CSL Behring is a global leader in the protein biotherapeutics industry, focused on bringing to market biotherapies used to treat serious and often rare conditions. CSL Behring operates CSL Plasma, one of the world's largest collectors of human plasma, which is used to create CSL’s therapies. Seqirus is one of the largest influenza vaccine companies in the world and is a transcontinental partner in pandemic preparedness and a major contributor to the prevention and control of influenza globally.

We invite you to take a look at the many career possibilities available around the globe and consider building your promising future at CSL by becoming a member of our team!

Job Description

Job Summary:

We are currently seeking a reliable and ambitious team player to join our Research Serology team. Selected individual will investigate the status of innate and adaptive immunity and assess the immunogenicity and efficacy of influenza vaccines, but not limited, in animal models and where appropriate in humans. This position supports the development of new and/or improved serological assays that will help differentiate novel influenza vaccines from current vaccines. The desired candidate will be able to work well with a diverse workforce within the Research Serology team as well as other teams in Seqirus’s Research and Science department as well as collaborators. Important aspects of this role include excellent organizational, multi-tasking and communication skills, along with the ability to efficiently thrive in a highly interactive and goal -and timeline-oriented team environment.

Major Accountabilities:

Optimize existing assays and development of new immunoassays for both preclinical and clinical testing of immune responses to novel influenza vaccine candidates.

  • Emphasis on the identification of serological markers to differentiate novel vaccines from existing vaccines.
  • Perform mammalian cell culture and independently develop, run, analyse and troubleshoot in vitro assays, such as cell-based activity assays.
  • Generate master and working stocks of virus and other microbial agents as directed that are well documented and characterized. Virology assays to include but not limited to virus propagation and virus titration by TCID50, focus forming assays and plaque assays.

Perform serology assays for serum samples from animal studies for the in vivo characterization of novel vaccine candidates.

  • Follow existing and/or design experimental protocols.
  • Prepare internal reports.

Support day-to-day operations of the laboratory

  • Ordering laboratory supplies, keeping laboratory inventory current, maintain laboratory safety protocols, develop standard operating procedures and manage laboratory databases.
  • Preparing common reagents, media and solutions.
  • Calibration, monitoring and cleaning of laboratory and lab equipment.

Demonstrate understanding, ownership, and accountability of assigned Research projects

  • Prepare reports as needed
  • Prepare and present data in the context of the overall project at cross-functional meetings
  • Conduct self-directed literature review to improve personal understanding and to troubleshoot & advance projects
  • Improve efficiency of methods and processes. 
  • Compose and revise protocols and technical documents. 

Function as a productive team member of the Seqirus Research group

  • Work and communicate effectively with others within and across functions
  • Demonstrate a high level of engagement and contribute to scientific discussions in meetings
  • Pursue cross-training opportunities or learn new technologies as needed to fulfill role
  • Train on and follow internal policies and procedures to maintain day-to-day lab operation, quality standards, HSE compliance, IP, performance management, etc.
  • Help build and promote a positive culture and working environment

Minimum Requirements:

REQUIRED:

  • BS/MS in relevant immunology and biology fields, or equivalent
  • BS with more than 4 years of pharmaceutical/biotech experience. MS with 1+ years of academic or pharmaceutical/biotech experience.
  • Strong critical, scientific, and strategic thinking skills allowing individual to work independently with minimal supervision
  • Knowledge and relevant laboratory experience in the fields of virology, immunology and cell biology:
  • Cell culture and aseptic technique
  • Advanced training in area of ELISA optimization  
  • Professional experience in the field of virology: virus production and characterization (virus growth, infectivity assays, virus titration and lot quality control studies)
  • Minimum of two (2) years of related experience in Biosafety Level 2 (BSL2) research environment.
  • Software skills: GraphPad Prism, Excel, PowerPoint
  • Strong written and verbal communication skills

PREFERRED:

  • 2+ years of hands-on experience in state-of-the-art serological techniques for influenza (HAI assay, MN and/or ELLA Assays).
  • Experience with molecular biology and biochemical techniques such as, immunoblotting techniques, PCR, qPCR, RT-PCR, DD-PCR, RNA/DNA extraction, nucleic acid purification, plasmid preparation, immunofluorescence, flow cytometry and microscopy
  • ELISpot, multiplex measurements (Luminex technologies). Experience in Cytation 5 or CTL.
  • Experience with documentation and reporting of results using electronic lab notebooks
  • Act as a leader in absence of supervisor