Research Associate II / Senior Research Associate I, Cell Culture

BioMarin is the world leader in delivering therapeutics that provide meaningful advances to patients who live with serious and life-threatening rare genetic diseases. We target diseases that lack effective therapies and affect relatively small numbers of patients, many of whom are children. These conditions are often inherited, difficult to diagnose, progressively debilitating and have few, if any, treatment options. BioMarin will continue to focus on advancing therapies that are the first or best of their kind.

BioMarin’s Research & Development group is responsible for everything from research and discovery to post-market clinical development. Research & Development involves all bench and clinical research and the associated groups that support those endeavors. Our teams work on developing first-in-class and best-in-class therapeutics that provide meaningful advances to patients who live with rare diseases. Come join our team and make a meaningful impact on patients’ lives.

We are looking for a highly motivated team member with extensive experience in mammalian cell culture models to be a key contributor to the Research Gene Therapy Group. The incumbent will be responsible for supporting the development of primary, iPSC-derived, and immortalized cell-based models for expression, safety, and functional testing of gene therapy candidates. The incumbent will also execute routine molecular and biochemical analyses and utilize novel state-of-the-art technologies like next-generation sequencing.

Responsibilities

• Perform routine cell culture as well as viral (AAV/LV) and non-viral modification of adherent, suspension, iPSC-derived, and primary cell lines
• Design, modify, and clone DNA constructs for expression of proteins and fusion proteins in mammalian expression systems
• Perform standard molecular biology techniques including DNA extraction, RNA extraction, and PCR/ddPCR
• Apply basic biochemical and immunological techniques, including protein extraction, protein gel electrophoresis, conventional and capillary western blotting, ELISA, and flow cytometry
• Perform routine molecular biology techniques using the engineered cell lines to support the development of new next-generation sequencing (NGS) assays for sequence determination, editing efficiency analysis, and off-target assessment
• Maintain detailed records of experimental protocols and data in an electronic laboratory notebook in accordance with company policy
• Effectively collaborate with a fully integrated research team to facilitate the success of projects
• Absent a Medical or Religious reason that prohibits vaccinations, all our incoming employees must be vaccinated

Qualifications

• BS or MS in molecular biology, biochemistry, synthetic biology, or relevant biological sciences
• Gene therapy or gene editing experience preferred, including working knowledge of state-of-the-art technologies utilized in the field.
• Strong technical background with standard laboratory methods, including culturing immortalized, iPSC-derived and primary cells; molecular biology and cloning; (q)PCR; ELISA; intracellular staining; western blotting; DNA transfection and viral transduction; flow cytometry or microscopy
• Ability to productively collaborate in cross-functional teams
• Excellent interpersonal communication and writing skills for presentations, electronic notebook, and report writing
• Ability to adapt to a new and fast-paced environment and navigate uncertainties
• Willingness to embrace new challenges, fearlessly innovate, and constantly learn

Level Differentiation

Research Associate 2:

• Bachelor’s degree and at least 4 years of experience; Master’s degree with at least 2 years of relevant experience

Senior Research Associate 1 :

• Bachelor’s degree and at least 6 years of experience; Master’s degree with at least 4 years of relevant experience