Associate Scientist, Protein Process Development & Manufacturing

Location
Foster City, California, United States
Posted
Jul 25, 2021
Ref
342434342
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
The Development organization is comprised of four Process teams: Protein, Polysaccharide,

Conjugate Drug Substance, and Drug Product. These Process Development teams are supported

by Analytical Development and Formulation Development teams.

Vaxcyte is looking for an Associate Scientist to join the Protein Process Development &

Manufacturing team to develop the downstream purification processes for multiple protein

antigens that are part of the VAX-A1 vaccine.

Essential Functions:

• Develop the downstream purification process for multiple protein antigens that comprise

the VAX-A1 vaccine; the purification process must be robust, scalable, and cost-effective

• Collaborate with Analytical Development to implement and run assays to assess product

quality and purity

• Generate material for analytical method and conjugation development

• Participate in cross-functional project teams and provide regular progress updates

• Participate in technology transfer activities for the process developed at Vaxcyte to an

external CMO for GMP production

Requirements:

• BS in Chemical Engineering, Biochemistry, or a related discipline, with 3+ years of

industry experience; MS with 1+ years of industry experience

• Strong protein purification knowledge and experience, including familiarity with AKTA

lab-scale and preparative chromatography systems and UNICORN software

• Experience with depth, nominal, and tangential flow filtration

• Hands-on experience purifying untagged proteins from crude lysate to high purity

• Solid understanding of the principals of DoE (Design of Experiments); practical experience

with DoE software

• Both practical experience with and theoretical knowledge of engineering principles

involved in scaling processes from development lab to pilot / manufacturing plant

• Working knowledge of the requirements of GMP manufacturing; hands-on GMP

experience a plus

• Experience working with CMOs highly desired; ability to travel to CMO (some

international travel required) to perform person-in-plant oversight activities

• Proficiency running gel- and column-based analytical methods a plus; such methods may

include SDS-PAGE, Western blotting, IEF, SEC, HIC, IEX, RP, Mass Spec, and binding

affinity or activity assays (such as MSD or ELISA)

• Ability and desire to learn upstream cell-free production system used to express proteins

•Practical experience with microbial fermentation and running bioreactors / fermenters (up

to 50 L scale) a plus

•Demonstrated success working in a cross-functional team environment on multiple

projects; ability to work both on a team and independently to deliver results

•Strong interpersonal skills, with excellent written and verbal communication skills