Associate Director, Nuclease Development

Brisbane, CA, United States
Jul 24, 2021
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time


Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences is advancing disease detection and treatment using its novel CRISPR systems that can deliver on the promise of precision medicine across diagnostics and therapeutics. Furthermore, Mammoth's CRISPR technology platform will enable new solutions in life science research, healthcare, agriculture, biodefense and more.

Based in the San Francisco Bay Area, Mammoth Biosciences is co-founded by CRISPR pioneer and Nobel Prize winner Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber.


Our small-size proteins, such as Cas14 and CasPhi, are poised to transform disease treatment enabling novel editing approaches and delivery options that are difficult to achieve or not even possible with previously described CRISPR-Cas systems. Our teams are responsible for discovering and engineering novel CRISPR systems, establishing CRISPR-based diagnostics, and translating our unique CRISPR technology into therapeutic treatments with the ultimate goal of cures for indications with high medical need.


Mammoth is seeking an Associate Director with expertise in mammalian-based CRISPR genome editing to join its Discovery team. Discovery is responsible for identifying and developing novel CRISPR-associated (Cas) nucleases for genome editing and diagnostic applications. This role will focus on nuclease development for editing applications.

The successful candidate will lead Mammoth's efforts to develop Cas nucleases for genome editing applications. The Associate Director of Nuclease Development will be situated between nuclease discovery and preclinical functional groups and will therefore have a central role in determining which genome editing nucleases are most suitable for specific indications. Success in this role will require a diverse range of experience that spans tissue culture, viral and nonviral delivery methods, protein engineering and laboratory automation.


    • Provide technical leadership for genome editing-facing nuclease development program
    • Screen and improve established genome editing nucleases for specific indications
    • Manage of individual contributors
    • Define the scope for protein engineering campaigns
    • Contribute towards shaping upstream nuclease discovery goals
    • Other duties as assigned





    • PhD
    • 6 years biotechnology industry experience
    • 2 years experience in Cas nuclease discovery or development





    • 3 years of technical leadership experience, managing Scientist- and Research Associate-level contributors
    • Experience with tissue culture for diverse immortalized cell lines
    • Hands-on experience with AAV, lentivirus, LNP, mRNA delivery approaches
    • Ability to design and execute NGS assays
    • Hands-on experience with laboratory automation (eg. liquid handlers, plate readers, automated colony pickers)
    • Deep familiarity with CRISPR-Cas literature
    • Computer and scripting skills (Benchling, Geneious, JMP, SQL, R, python)





    • Company-paid health/vision/dental benefits
    • Unlimited vacation and generous sick time
    • Company-sponsored meals and snacks
    • Wellness, caregiver and ergonomics benefits
    • 401(k) with company matching


It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation.