Research Associate, CNS Stem Cell Culture

Location
Cambridge
Posted
Jul 14, 2021
Ref
5080621002
Discipline
Science/R&D, Research
Required Education
Bachelors Degree
Position Type
Full time
JOB PURPOSE

The Research Associate, CNS Stem Cell Culture is responsible for developing as well as executing novel protocols for neural and glial differentiation in Sana's Cambridge CNS labs. This individual will execute the cell culture operations of Sana's CNS Therapy lab, with direct oversight of at least one of its experimental programs. This position includes technically diverse bench work as well as the training of research staff in techniques and equipment use, across the CNS in vitro program.

The Research Associate, CNS Stem Cell Culture will report on a direct basis to the Scientist, CNS Cell Therapy, and work under the broader direction of the Director, CNS Cell Biology and the Head of CNS Therapy.

DUTIES AND RESPONSIBILITIES
  • Assuring the quality and integrity of all data collection and documentation
  • Expansion and cryopreservation of pluripotent stem cells
  • Generation of neural and glial lineages from pluripotent stem cells
  • Performance and supervision of flow cytometry and cell sorting
  • Performance and supervision of routine imaging and image documentation of cells
  • RNA and DNA isolation and preparation for genomic analyses.
  • Daily management of the cell culture lab as needed
  • Personnel direction and training
  • Assuring compliance with all relevant lab safety and biosafety regulations
  • Demonstrate Sana's core values and the attributes that support these core values (Lead from every seat, Thrive as a team, Make it happen)

QUALIFICATIONS

Basic Qualifications
  • BA/MA or MS in neuroscience, cell biology, or biotechnology
  • Extensive hands-on expertise in pluripotent stem cell biology and stem cell culture techniques
  • 2-3+ years of post-graduate academic or industry experience in neural and glial cell biology
  • Significant expertise in and understanding of stem cell biology
  • Significant experience with immunochemistry, digital image analysis, and confocal microscopy
  • Excellent interpersonal, organizational, and independent problem-solving skills
  • Proficiency with standard cytometry and imaging software (e.g., FACSDiva, FlowJo, ImageJ, etc.)
  • Must be an effective communicator both verbally and in writing
  • Working safely in person on a predictable and regular in-person basis is an essential job function for this position and the Company requires that all individuals working in-person, subject to certain exceptions, be fully vaccinated against COVID-19

Preferred Qualifications
  • Experience in deriving both neuronal and glial cells from pluripotent stem cells
  • Experience in making clonal lines of pluripotent stems cells
  • Experience in genome editing techniques (ie CRISPR)
  • Neuroscience background

WORKING CONDITIONS
  • Scheduled weekend and holiday coverage required for culture maintenance and analysis, in rotation with supervised technical staff, to ensure successful project performance with sampling at defined time points.


To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The job description listed above is representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

At Sana, we believe that a diverse workforce strengthens us as a company and helps us to achieve our mission to meaningfully change the outcome of many human diseases. This belief is a pillar of our business and is critical to our success. Our goal is that Sana is the place for talented people to bring their authentic selves to work, to have a great career and to deeply, positively impact patients.

The Company is committed to providing a workplace free of discrimination and harassment based on race, color, religion, age, gender, national origin, ancestry, physical or mental or sensory disability (including the use of a trained guide dog or service animal by a person with a disability), genetic information, actual or perceived HIV or Hepatitis C infection, medical condition (cancer or a record or history of cancer and genetic characteristics), marital status, sexual orientation, sexual preference, pregnancy (including childbirth and related medical conditions), disability, veteran status, political ideology, taking or requesting statutorily protected leave, status as a victim of domestic violence, sexual assault or stalking, or any other basis prohibited by applicable law.

Core to our values, we believe there is nothing more important than the health and wellness of you and your family. We cover 100% of the cost for employee health coverage and offer generous time-off, parental leave, short and long term disability, financial wellness programs, a 401(k) Plan, Employee Stock Purchase Plan, commuter subsidy and a variety of wellness offerings to support each person individually.