Senior Scientist, Cell & Gene Therapy Technologies

London, United Kingdom
Jul 10, 2021
Required Education
Bachelors Degree
Position Type
Full time
Orchard Therapeutics, Ltd. (OTL) is looking for an experienced Senior Scientist to develop and optimize lentiviral vector (LVVs) platform(s). This is a great opportunity to join the growing Process Development team within Cell & Gene Therapy Technologies department and contribute to the delivery of Orchard Therapeutics innovative gene-modified cell-based therapeutic products. The job role will include molecular biology, cell line development, process development, process characterization and manufacture of lentiviral vectors. The post-holder will also input into the transfer and implementation of optimized processes to GMP to support OTL's product pipeline programs.

Key Elements and Responsibilities
  • Develop stable cell lines for LVVs;
  • Design, develop and improve process(es) (USP and DSP) for LVVs to deliver robust manufacturing platforms for early and late-stage clinical and commercial development;
  • Develop and perform cell-based and biochemical assays to characterise LVV processes and products;
  • Execute experimental plans, analyse and interpret experimental data, maintain experimental results in relevant databases and write technical reports;
  • Provide hands-on technical expertise/scientific guidance to team members and supervision of junior colleagues;
  • Contribute to the routine lab maintenance;
  • Contribute to the transfer of stable cell lines and improved processes to GMP and assist with oversight of manufacturing activities as required;
  • Prepare, review and complete protocols/SOPs, study reports and assist with regulatory submissions;
  • Communicate, share and discuss work progress internally and externally;
  • Other responsibilities may be assigned.


Required knowledge
  • In-depth knowledge on the state-of-the-art of cell & gene therapy products and available technology, more specifically regarding viral vectors;
  • PhD+3 years (academic/industrial) or additional industrial experience with development, manufacture and delivery of viral vectors, preferably retro and lentiviral vectors;
  • Demonstrated skills and hands-on experience in tissue culture techniques and handling of adherent and suspension cell lines in different culture systems;
  • Demonstrated skills and hands-on experience in molecular biology and vectorology, including cloning, standard DNA and RNA technology, analyses with qPCR, recombinant protein expression and assessments with SDS-PAGE, Western blot, ELISA, flow cytometry and others;
  • Demonstrated knowledge and hands-on experience with stable cell lines development for retro and lentiviral vectors;
  • Demonstrated knowledge in designing and developing processes for LVV production and purification;
  • Experience in developing and performing analytical methods for stable cell lines and LVV characterization;
  • Use of more specialist lab equipment e.g. flow cytometers, cell counters, cell sorters, microscopes and imaging systems, bioreactors, AKTA, TFF systems, controlled-rate freezers etc;
  • Proficient with MS office, Prism, SnapGene, FlowJo, e-lab books and other commonly used software;
  • Experience with DoE software (s) and statistical analyses is a plus;
  • Understanding of and familiarity with regulatory guidance governing stable cell lines and vector-based products is a plus;

Skills & Abilities
  • Ability to work collaboratively as part of a multi-disciplinary team, or independently as necessary;
  • to prioritise work and meet deadlines;
  • Independently design and execute experiments following laboratory procedures, appropriately analyse data and provide an interpretation of results;
  • and written skills for report writing, team communications and presentations;
  • Creativeness, motivation, and troubleshooting skills to solve problems and/or develop new technical capabilities;
  • Ability to operate in a fast paced, multidisciplinary industrial environment

  • A minimum PhD or equivalent in biotechnology, process development, cell line development, cell and gene therapy, biological sciences, or other relevant scientific discipline.