R&D Specialist (In Vivo - Viral Vector Technologies)

Location
Tarrytown, New York, United States of America
Posted
Jul 04, 2021
Ref
25079BR
Required Education
Bachelors Degree
Position Type
Full time
Regeneron is currently seeking a talented and motivated R&D Specialist to join a technology development group which aims to develop novel viral vectors. The candidate's main goal will be to design and produce viral vectors, and to

characterize them by performing various in vitro and in vivo assays to measure their infectivity and ability to transduce target cells of interest. Projects will

involve using various mouse models and a variety of assays to monitor transduction, such as bioluminescent imaging, immunohistochemistry, in situ hybridization, vector genome quantification from tissue, flow cytometry, and ELISA.

As an R&D Specialist in Viral Vector Technologies, a typical day may include the following:

- Maintain, transfect, and transduce mammalian cell cultures
- Produce, purify, quantify, and characterize viral vectors in BLS2 cell culture for in vivo studies
- Dose mice with viral vectors and/or other therapies via tailvein (IV) injection, IP, IM, or SubQ routes
- Monitor infection/biodistribution of viral vectors through in vivo bioluminescent imaging, ex vivo fluorescence imaging, immunohistochemistry, in situ

hybridization or by blood draws and serum/plasma collections
- Collect biological samples from mice through dissections; process samples appropriately for downstream analysis (immunohistochemistry, qPCR, RNAScope/BaseScope, flow cytometry)
- Assess non-human primate biological samples (PBMCs, plasma/serum, FFPE tissue)
- Perform basic biochemical/immunological assays (ELISA, flow cytometry, Luminex/MSD)
- Perform basic molecular biology assays (Cloning, Western blotting, DNA/RNA extractions, PCR, RT-PCR, quantitative PCR, etc.)
- Analyze and present data (familitarity with Graphpad Prism or other statistical/graphical software)
- Maintain lab supplies and help with lab organization

This role may be for you if:

- You enjoy working in a fast-paced team environment and collaborating with others on exciting projects
- You are able to handle multiple projects and are comfortable changing gears quickly as needed

To be considered for this role, you must have a BS/MS with 3 + years of experience. Experience with animal handling, animal injections (specifically tailvein), bleeds (retro-orbital, tail-nick, etc.) is required. Experience with serum/plasma collection, bioluminescent imaging, mouse dissections and tissue analysis by various methods is required. Experience with mammalian cell culture, molecular biology, and biochemistry techniques is preferred. Experience working with Adeno-associated virus (AAV) or CRISPR/Cas9 is a plus.

Does this sound like you? Apply now to take your first steps toward living the Regeneron Way! We have an inclusive and diverse culture that provides amazing benefits including health and wellness programs, fitness centers and stock for employees at all levels!

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion or belief (or lack thereof), sex, nationality, national or ethnic origin, civil status, age, citizenship status, membership of the Traveler community, sexual orientation, disability, genetic information, familial status, marital or registered civil partnership status, pregnancy or maternity status, gender identity, gender reassignment, military or veteran status, or any other protected characteristic in accordance with applicable laws and regulations. We will ensure that individuals with disabilities are provided reasonable accommodations to participate in the job application process. Please contact us to discuss any accommodations you think you may need.