Scientist II, Analytical Sciences - Gene Therapy Early Pipeline

Location
San Diego, CA, US
Posted
Jun 30, 2021
Ref
6153
Hotbed
Biotech Beach
Required Education
Bachelors Degree
Position Type
Full time
Overview

Imagine\u2026 2 weeks after the birth of your child you realize they aren't meeting critical developmental milestones and after months of doctor's appointments you are told your baby has a rare neurological disease that would impact how they would live their lives. This is the reality of parents whose children have Spinal Muscular Atrophy (SMA) and it's the reason why we at Novartis Gene Therapies are laser focused on bringing hope and possibility to those devastated by rare genetic diseases.

The Scientist II, Analytical Sciences - Gene Therapy Early Pipeline, supports early-stage recombinant adenovirus-associated virus (rAAV) gene therapy pipeline portfolio.

Responsibilities
  • Develops and optimizes methods for extraction of DNA, RNA and proteins followed by testing using various molecular biology techniques such as qPCR, PCR, qRT-PCR, Immunofluorescence, Western blotting and ELISA to support characterization of novel AAV gene therapy vectors and cell line development.
  • Develops and executes ddPCR and ELISA assays for genomic titer, capsid titer and other testing's as required to enable progression of early pipeline development projects in timely fashion.
  • Supports or contributes to development or optimization of new analytical methods for characterization of gene therapy drug products and drug substances.
  • Develops vector lot release testing strategy and review all testing documents and data in preparation of vector release (COA) as part of the early pipeline project deliverables.
  • Performs a wide variety of laboratory tasks and experiments.
  • Maintains electronic laboratory notebook that records all aspects of experimental design and results, following good documentation practices.
  • Critical thinking, excellent data analysis using software's such as Microsoft Excel, JMP, Prism, R scripts and other software as needed with minimal supervision and interpretation skills.

Qualifications
  • Bachelor's in Analytical Chemistry, Molecular Biology, Cell Biology, Biochemistry, Virology, or a related field with 6 years' relevant experience or Master's with 4 years relevant experience or PHD with 2 years relevant experience.
  • Extensive experience in analytical chemistry and molecular biology.
  • Passionate about new analytical technologies/methods to assess the quality of research vectors, including characterization and release assays for early pipeline development projects.
  • Familiarity with standard analytical techniques for characterizing rAAV Vectors (SEC, AUC, Purity, CE, RP-HPLC, DLS, LC-MS etc).
  • Highly organized, attentive to detail, and able to work under tight timelines in a collaborative team setting.
  • Excellent interpersonal and communication skills.
  • Strong hands-on experiences on analytical techniques such as RT-PCR, qPCR, ddPCR, Immunoassays such as ELISA, SDS-PAGE, and Western blot.
  • Hands-on experiences on variety of biochemical and biophysical assays including HPLC (SEC, IEC, RP), osmolality, Analytical ultra-centrifugation (AUC), Light Scattering (SLS, DLS & MALS), capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and UV-Vis Spectrometry.
  • Ability to troubleshoot and think analytically, multitask and support multiple projects simultaneously.
  • Quick learner, highly motivated, hard-working and detail oriented.


The level of this position will be based on the final candidate's qualifications.

Why Novartis?

769 million lives were touched by Novartis medicines in 2020, and while we're proud of this, we know there is so much more we could do to help improve and extend people's lives. We believe new insights, perspectives and ground-breaking solutions can be found at the intersection of medical science and digital innovation. That a diverse, equitable and inclusive environment inspires new ways of working.

We believe our potential can thrive and grow in an unbossed culture underpinned by integrity, curiosity and flexibility. And we can reinvent what's possible, when we collaborate with courage to aggressively and ambitiously tackle the world's toughest medical challenges. Because the greatest risk in life, is the risk of never trying! Imagine what you could do here at Novartis!

Novartis Gene Therapies Benefit Summary:

Annual bonus, long term incentive, health insurance, paid vacation/holidays, potential flexible working arrangements (based on role) and an employee recognition program are available for this position, among many other benefits provided to employees of Novartis Gene Therapies.

Please note this job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required of the employee for this job. Duties, responsibilities and activities may change at any time with or without notice.

Novartis Gene Therapies is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity, national origin, genetics, disability, age, sexual orientation or veteran status.

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