Scientist, Payload Enginering
- Employer
- 4D Molecular Therapeutics
- Location
- Emeryville, CA
- Start date
- Jun 16, 2021
View more
- Discipline
- Science/R&D, Biotechnology
- Required Education
- Bachelors Degree
- Position Type
- Full time
- Hotbed
- Biotech Bay
Job Details
4DMT is focused on attracting and retaining the best people in the industry who identify with our mission and innovative results-driven approach. We offer an inspiring and collaborative environment that encourages individual contributions, recognizes performance in multiple ways, offers competitive salary and benefits, and provides challenging opportunities for career development. 4D Molecular Therapeutics is located in Emeryville, CA.
GENERAL SUMMARY:
This role supports the Payload Engineering group within the Vector and Product Development Unit. They will report to a Scientist within the Payload team and work in collaboration with their manager and other research associates and scientists on the team and within the greater V&P unit to design, construct and validate function of AAV cassettes for various disease indications. They will play an integral part in building novel therapeutics for a range of disease targets utilizing a variety of intervention strategies including, gene replacement, siRNA and antibody production.
Responsibilities:
- Lab Activities: 70%
- Supports the design and construction of AAV genomic DNA cassettes through molecular DNA cloning techniques
- Amplify and purify DNA plasmids
- Development of assays to test expression and function of constructed plasmids Communication and Scientific Administrative Tasks: 30%
- Contribute scientific ideas and knowledge in departmental and cross-functional team meetings
- Effectively present findings at departmental and cross-functional team meetings
- Engage in study design for various programs
- Help compile and write data sections for IND preclinical pharmacology reports
- Communicate effectively with management to achieve departmental goals
- Other duties as assigned, nothing in this job description restricts management’s right to assign or reassign duties and responsibilities to this job at any time
Qualifications:
- Ph.D. in relevant field, Cell and Molecular Biology or Master’s Degree in relevant field – 2+ years of relevant experience Experience:
- 4+ years of molecular cloning experience within the Biotech or similar industry
- 2+ years basic cell culture experience Skills:
- Molecular DNA cloning
- PCR and other DNA amplification and isolation techniques
- Cell Culture
- Transformation/Transfection/Transduction
- Gel electrophoresis and DNA purification
- ELISA/Western blot analysis
- Sequencing analysis and plasmid map design
- Applies technical and functional knowledge to design experiments / projects that contribute to the departmental goals
- Guides the successful completion of projects in collaboration with team members
- Demonstrated success in technical proficiency, scientific creativity, collaboration with others and independent thought
- Exercises judgment within broadly defined practices and policies in selecting methods, techniques, and evaluation criteria for obtaining results
- Applies scientific/technical knowledge gained at conferences or through literature to advance project work
- Acts as a resource for colleagues with less experience; may lead small projects with manageable risks and resource requirements
- Contributes to and may author scientific/technical documents (posters, publications, regulatory documents, patent applications, technical reports)
- Effective communication and collaboration within team and across departments Physical Requirements of the Role:
- Lifting no more than 20 lbs for lab organization and storage
- During COVID-19 will this person need to be in a lab 5 days a week and adhere to 4DMT Covid protocols and policy
4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
Company
4DMT is a gene therapy company with a transformative discovery platform --Therapeutic Vector Evolution --that enables our “disease first” approach to product discovery and development, thereby allowing us to customize our AAV vectors to target specific tissue types associated with the underlying disease.
At 4DMT we are creating and developing optimized “biopharmaceutical grade” AAV vectors that will allow us uniquely to unlock the full potential of gene therapy. Our customized and proprietary AAV vectors are each designed, according to a 4DMT Target Vector Profile, to deliver genes to specific cells and tissues in the body to eradicate both rare and large market diseases.
While AAV gene therapy pioneers are making great strides with the first wave of products, hurdles still exist with the use of conventional vectors. The most commonly used AAV capsids (e.g., AAV2, 5, 8 and 9) were identified either 1) as contaminants in lab stocks of adenovirus, or 2) through monkey tissue processing. Therefore, these conventional vectors are not targeted specifically to any tissue in the body. This can lead to inefficient and non-specific delivery, thus requiring extremely high doses and potentially resulting in toxicities (including inflammation), high manufacturing burdens and suboptimal efficacy. In addition, suboptimal routes of delivery can be required for delivery to the right tissues (e.g., subretinal delivery to the retina). Finally, pre-existing neutralizing antibodies in many patients can limit efficacy, clinical trial enrollment and market sizes. 4DMT Innovation empowers us to create new and improved next generation AAV capsids to allow targeted delivery of our products by the optimal clinical route of administration.
At 4DMT we use our robust discovery platform, termed “Therapeutic Vector Evolution,” to create customized and proprietary gene delivery vehicles (novel AAV vectors) to deliver genes to specific tissues and cells in the body by the optimal route of administration. We use the power of evolution to create and refine these optimized vectors through genetic diversification and then natural selection in vivo.
4D Purpose Statement: Boldly Innovating to Unlock the Full Potential of Gene Therapy for Countless Patients
GUIDING PRINCIPLES
- Dare to Cure - Patients are waiting. Create big dreams for patients and take calculated risks to achieve them
- Break Boundaries - Question the status quo and innovate beyond conventional approaches
- Beyond Yourself - It's about patients, their families, our team, our families. We strengthen each other.
- Prepare and Execute Relentlessly - Start with the end in mind, over-prepare and make contingency plans to your contingency plans
We invite all of you who share our passion and vision in creating the ultimate gene therapy products to cure genetic and rare diseases in children and adults. We seek the best people in the industry who identify with our mission and innovative results-driven approach. We offer an inspiring and collaborative environment that encourages individual contributions, recognizes performance in multiple ways, offers generous benefits and provides challenging opportunities for career development.
- Website
- http://www.4dmoleculartherapeutics.com/
- Phone
- 510-505-2680
- Location
-
5858 Horton St. Suite 455
Emeryville
California
94608
United States
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