Associate Scientist, Cell Line Development

Location
Gaithersburg, MD, United States
Posted
May 30, 2021
Ref
2021-10139
Hotbed
BioCapital
Required Education
Bachelors Degree
Position Type
Full time
Overview

We are currently searching for an Associate Scientist, Cell Line Development to provide support to the National Institutes of Health (NIH). This opportunity is a full-time position with MSC, and it is on-site in Gaithersburg, MD.

Duties & Responsibilities

  • Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
  • Independently execute each step of the cell line development process including plasmid production, transfection, single cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.
  • Use automated platforms for clone selection and screening.
  • Prepare plasmid DNA using standard molecular cloning techniques.
  • Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
  • Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
  • Generate stable pools and perform transient protein production for supply of reagent-grade material
  • Provide laboratory support for medium preparation and ordering of supplies.
  • Maintenance of ambr15 high-throughput microbioreactor systems and associated bioprocess analyzer, coordination of service visits, and regular ordering of consumables.
  • Train other laboratory personnel on use of equipment and corresponding processes.
  • Write and review technical reports documenting process development studies and stable cell line generation work.
  • Work with other team members to generate experimental plans for process development studies.
  • Write and review standard operating procedures for cell line development processes and instrumentation.
  • Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
  • Regularly communicate progress to team lead and present work to cell line development team.


Requirements

  • B.S. in Biological Sciences with a minimum of 5 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting.
  • Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry.
  • Experience in preparing technical reports, standard operating procedures, and data presentations.
  • Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines.
  • Ability to effectively communicate plans, results, challenges, and ideas to other team members in oral and written forms.
  • Familiarity with computer software for word processing and data analysis.
  • Experience in monoclonal antibody and/or vaccine production.

#LI-SK1

Company Description

We are a trusted government partner that blends deep domain expertise with advanced technologies to help our customers solve complex problems that improve, protect, and save lives. As a rapidly growing company, we combine entrepreneurial spirit, customer focus, and an outcomes-based approach to support agency missions in health IT, life sciences, public safety, and grants management.

The Dovel Family of Companies offers employees an opportunity to advance beyond a specific role or contract, we offer a path to develop an enriching career. We believe in empowering a culture of innovation, customer success, and employee growth.

What you'll get...
  • Time Off! Flexible schedules and company paid holidays allow you to take the time you need.
  • Investment in YOU! 401(K) company contributions are yours to keep with no waiting period.
  • Choices! Unique healthcare plans to choose from with options like fertility and orthodontia benefits.
  • Discovery! With our tuition assistance and training programs, we support your career advancement.
  • Tax Savings! Enroll in pre-tax Health or Dependent Care Flexible Spending, HSA with company contributions, parking, and/or transit commuter benefits.
  • Support! Working parents and busy professionals - we've got you covered with a supportive culture, confidential Employee Assistance Program and a membership to Care.com.
  • Perks! Employee discounts, peer recognition programs, company-wide wellness challenges, and fun community events.
  • A Voice! A unique culture where you can influence decisions and have your voice heard.


We are an Equal Opportunity Employer with a commitment to diversity. All individuals, regardless of personal characteristics, are encouraged to apply. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, sexual orientation, gender identity, disability, or veteran status.