TD Principal Scientist/Engineer I, Molecular & Cell Biology

Location
Redwood City, CA
Posted
May 28, 2021
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Adverum is looking for a highly motivated Principal Scientist/Engineer I to lead the Molecular & Cell Biology function for the Candidate Process Development (CPD) team at our Redwood City, CA office. The successful candidate will play a critical part in the development of a state-of-the-art molecular biology toolbox and its application to Adverum’s gene therapy pipeline of products. The incumbent will be responsible for evaluating and developing latest molecular biology techniques to support AAV gene therapy pipeline and implement the operational vision for CPD group. You will independently design and conduct experiments for multiple aspects of AAV Gene Therapy projects including but not limited to Molecular Cloning, DNA synthesis, vector design, alkaline gel electrophoresis, codon optimization and application of ddPCR, qPCR techniques, NGS and sequence analysis. This role reports into the Director, Head of Candidate Process Development and will be expected to work independently, manage projects, manage a small team of scientists and participate in cross-functional, multidisciplinary project teams.
 
This role requires strong collaborative Team focus, the ability to juggle immediate needs with long term goals, and an ability to be highly productive in a fluid, fast-paced and teamwork-oriented environment. Direct experience of AAV biology and relevant molecular biology methods is a plus.
What You'll Do
  • Evaluate and implement state of the art molecular biology technologies and workflows to support the vector supply for pre-clinical and clinical manufacturing of adeno-associated viral (AAV) vectors for Gene Therapy
  • Independently design and execute experiments to generate a molecular biology tool box to synthesize, optimize and analyze AAV vectors, transgene and critical reagents.
  • Optimize AAV platforms by Bacmid/Baculovirus genomic engineering
  • Assemble potential therapeutic reagents into plasmids or other vectors by performing molecular cloning protocols including Gibson assembly, Golden Gate reactions and site directed mutagenesis etc.
  • Collaborate with other departments such as Research, PD, Analytical development, and manufacturing sciences to understand the requirements and support them.
  • Accurate documentation for experiments with regular updates to the internal & external teams
  • Write and review high quality technical development and technology transfer reports
  • Serve as a subject matter expert (SME) for AAV molecular biology methods and techniques to internal and external partners.
  • Manage 1-2 direct reports
  • Participate in scientific conferences and make contributions to scientific publications and patents

About You
  • PhD in Molecular Biology, virology or related biological sciences with 7+ years of relevant experience, Master’s/ Bachelor’s degree with a minimum of 12+ years of relevant industry experience. 
  • Strong expertise in molecular cloning of vector plasmids (restriction digestion, Gibson assembly, ligation and screening), experience with AAV vectors & transgene expression plasmids a plus. 
  • Expertise in advanced molecular biology & related lab techniques (ddPCR, qPCR, SDS-PAGE, westerns, ELISAs, etc.). 
  • Skilled with primer design, Sanger sequencing and sequence analysis software. 
  • Experience with next generation sequencing (Illumina or PacBio platform) and NGS related data analysis is desired 
  • Good knowledge of principles and techniques in AAV biology and molecular virology. 
  • Hands-on experience with mammalian and sf9 insect cell culture (adherent and suspension cells) and viral vector production is desirable but not required 
  • Ability to design complex cloning strategy independently with passion to train junior team members 
  • Ability to interpret results analytically and troubleshoot technical problems 
  • Excellent communication and presentation skills 
  • Excellent organization and attention to detail; critical and creative thinker. 
  • Able to work in a collaborative / team-oriented environment and commit to internal documentation practices.

About Us
Adverum is a clinical-stage gene therapy company targeting unmet medical needs in ophthalmology and rare diseases. Adverum develops gene therapy product candidates designed to provide durable efficacy by inducing sustained expression of a therapeutic protein. Adverum’s core capabilities include clinical development, novel vector discovery and in-house manufacturing expertise, specifically in scalable process development, assay development, and current Good Manufacturing Practices quality control.

At Adverum, Inclusion and Diversity are at our core. We believe in the power of being your authentic self.  We strive to create the space which allows for everyone in our Adverum Community to not only feel safe but encouraged to speak, learn from each other, grow in their professions and be the very best versions of themselves no matter what their age, ethnic background, gender, origin, religion or sexual orientation