Senior Scientist - Protein Engineering

Location
South San Francisco, CA
Posted
May 18, 2021
Required Education
Masters Degree/MBA
Position Type
Full time

TRex Bio is an advanced, cutting edge biotech company focused on advancing first-in-class therapeutics to treat immune-inflammatory and immune-oncology indications by modulating tissue-specific regulatory T (Treg) and other immune cells. 

The Role  

TRex Bio is looking for an experienced and motivated senior scientist to join our growing biotherapeutic development team. The candidate will drive early discovery research programs into clinical development, providing scientific expertise in generation of protein reagents, antibodies, engineering, purification and biophysical characterization. A strong candidate will drive laboratory bench work, most critically production and purification of proteins, as well as subsequent biophysical characterization.  The candidate will join a dynamic, supportive, and motivated team, and work with a range of scientists company-wide to advance novel therapeutic projects. The candidate will report directly to the Head of Biotherapeutics and will play a key role in leading the Protein Engineering group and its functionality, with the expectation of leading junior associates as the company and Protein Engineering group grows. 

We are looking for candidates who can:  

  • Perform and supervise mammalian and bacterial cell culture work to support transient protein production, including relevant molecular biology, cloning, transfection and cell culture activities 
  • Perform and supervise basic protein purification, quantification and characterization assays 
  • FPLC-based purification of proteins 
  • HPLC-based protein analytical characterization 
  • UV spectroscopy, SDS-PAGE, western-blot analyses 
  • Protein labeling/conjugation reactions 
  • Direct and analyze protein-ligand binding kinetics and affinity by Biacore (SPR), Octet, KinExA and related technologies 
  • Direct and analyze protein stability and aggregation properties using DSC, UNCLE, Thermocycler, DLS, HIC, PSR and related assays performed in-house or at CROs 
  • Lead implementation and execution of software tools for protein DNA and protein reagent tracking and inventory across projects and the company 
  • Work with the Head of Biotherapeutics to analyze antibody sequences, perform antibody engineering as needed, bringing to bear necessary resources and tools for sequence analysis and design to advance lead candidate therapeutics 
  • Design and provide needed day-to-day oversight for CRO based projects in the area of protein science, protein production, developability and characterization 
  • Lead multi-departmental projects and discussions, and communicate results to group leaders and associates clearly and effectively 
  • Lead the development of methodologies, experimental platforms and procedures to expand protein science functionality 
  • Take lead role in solving problems, providing direction and implementing methods that impact project goals and success 
  • Prepare and present information from seminars and literature for group meetings 
  • Troubleshoot equipment and assay problems quickly, and optimize procedures and protocols to improve experimental throughput 

The Candidate 

  • PhD/MS in biochemistry, biology, immunology, or similar field plus 5-8 years industry experience 
  • Strong expertise in at least one of the following areas: 
  • Protein and antibody therapeutic, discovery and development 
  • Broad expertise in a wide spectrum of biophysical assays used for protein characterization  
  • Protein engineering and design 
  • Mammalian cell culture and production 
  • Proficiency in data analysis software such as MS Excel, Graphpad Prism, etc.  
  • Ability to perform complex data analysis, and to analyze and interpret work published in the field   
  • Strong presentation and communication skills (oral and written)  
  • Ability to see ‘the big picture,’ contextualize problems, and find solutions  
  • Driven by innovation and motivated by the identification of novel targets for diseases with high unmet medical needs