Scientist, Cell Engineering

Location
South San Francisco, CA, United States
Posted
May 02, 2021
Ref
4828201002
Required Education
Bachelors Degree
Position Type
Full time
JOB PURPOSE

Sana Biotechnology is developing advanced delivery and genome engineering technologies to enable the next generation of cell and gene therapies. We are looking for a highly motivated and creative Scientist to join our new Cell Engineering group. The successful candidate will work with colleagues to establish novel genome editing capabilities and methods that will be used to develop engineered cells for our therapeutic programs. The environment is exciting and fast-paced and offers the opportunity to work and publish at the cutting edge of gene editing research.

DUTIES AND RESPONSIBILITIES
  • Identify reagents for efficient single and multiplexed gene editing to create Sana cell and gene therapies
  • Develop methods for the efficient delivery of gene editing reagents to diverse cell types for ex vivo and in vivo applications
  • Work with the group to establish and apply novel cell engineering capabilities
  • Coordinate with cross-functional teams to expand the range and sophistication of engineerable behaviors that can be applied to our cell therapy programs
  • Generate knock-out and knock-in critical cell lines using gene editing tools
  • Verify editing via sequencing methods (Sanger/NGS) and functional assays such as flow cytometry, western blotting, qRT-PCR, microscopy
  • Work closely with development teams to adapt reagents, assays and methods to clinical manufacturing
  • Assist in production and purification of viral vectors
  • Communicate and document findings via verbal and written methods

QUALIFICATIONS

Basic Qualifications
  • PhD +2 years of academic/industry experience or BA/MS +7 years academic/industry experience
  • Proficiency in the culture and editing of diverse mammalian cell types
  • Strong scientific background and publication record with proven high levels of performance
  • Excellent communication skills; ability to present effectively to teams with diverse backgrounds
  • Ability to adapt to increasing scope and complexity of work as projects mature
  • Comfortable in a fast-paced research environment with minimal direction, and able to adjust workload based upon changing priorities

Preferred Qualifications
  • Experience in the production of viral vectors including lentivirus and AAV
  • Familiarity with viral vector titering methods including ELISA for viral proteins like p24 and flow cytometry to measure transduction of target cells with GFP
  • Demonstrated high throughput and multiplexing capabilities
  • Expertise in multicolor flow cytometry and FACS sorting
  • Experience with assays for measuring cellular DNA damage response
  • Molecular biology skills