Associate Scientist, Assay Development Molecular Biology (yl)

Location
Redwood City, CA
Posted
Apr 18, 2021
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Adverum is looking for an Associate Scientist with knowledge of molecular biology to join our team in our Redwood City office. This position reports to a Scientist/Team Leader.  This person will be working with Assay Development, QC, and Process Development.  This role is responsible for developing, qualifying, transferring, and troubleshooting molecular biology assays in support of product characterization/release and bioanalysis.
What you'll do:
  • Work with senior scientific personnel to design, develop, qualify, troubleshoot, and transfer molecular biology assays to internal and external scientists in some subset of the following categories: 
  • AAV titer assay by qPCR/ddPCR in different stages of vector processing
  • Host cell residual DNA assay
  • Viral/plasmid residual DNA assay
  • Nucleic acid extraction
  • K/biodistribution assay to measure DNA/RNA in biological samples
  • Capillary Electrophoresis assays
  • Product characterization assays
  • Participate in decisions on experimental approaches and interpretation of data.
  • Work collaboratively with different research and development departments to evaluate, develop, and implement new assay formats and/or technologies.
  • Perform internal sample analysis as needed in support of product characterization and preclinical studies.
  • Transfer assays and oversee work at Contract Research Organizations and in the QC group.
  • Maintain current knowledge of regulatory requirements for bioanalytical and product characterization assays.
  • Write test procedures, documented standard test methods, SOPs, protocols, and technical reports.
  • Present project status updates in internal and external forums.
  • Maintain excellent documentation of laboratory activities.
  • Perform routine lab maintenance.
  • Other responsibilities may be assigned as needed.

About you:
  • Minimum of a biological science or similar Bachelor’s degree with 6-8 years’ of relevant experience in a biotech company, or a Master’s degree with 4-5 years’ of relevant experience, or an equivalent combination of education and experience.
  • Experience in assay development is required.
  • Ability to read and extract information from published literature and internal reports is required.
  • Experience in the qualification/validation of quantitative assays and knowledge of FDA requirements is desired.
  • Experience with cell culture, cell-based assays, viral transduction is required
  • Experience with AAV TCID50 is highly desired
  • Experience with qPCR and qPCR optimization, sample prep with automation, Quantstudio/ddPCR instruments, understanding of AAV biology is desired
  • Experience with transfer of assays to QC groups or CROs.
  • Experience with MSD, Luminex assays is a plus.
  • Experience with immuno-PCR is a plus.
  • Must be a self-starter, proactive, co-operative, and proficient at working both independently and in a small team environment.
  • Proven initiative and demonstrated accountability in a fast-paced environment with high degree of flexibility.
  • Detail oriented with time management skills.
  • Excellent verbal and written communication skills.
  • Experience in presenting data through written reports and oral presentation.
  • Publication in a peer-reviewed journal or presentation at a conference is desired.
  • Proficiency with GraphPad Prism, JMP, SoftMaxPro, and other common analytical software is desired.
  • Experience with bioinformatics with Genbank and application to qPCR assay design is a plusProficient with MS Office and other common office software.

About Us
Adverum is a clinical-stage gene therapy company targeting unmet medical needs in ophthalmology and rare diseases. Adverum develops gene therapy product candidates designed to provide durable efficacy by inducing sustained expression of a therapeutic protein. Adverum’s core capabilities include clinical development, novel vector discovery and in-house manufacturing expertise, specifically in scalable process development, assay development, and current Good Manufacturing Practices quality control.

At Adverum, Inclusion and Diversity are at our core. We believe in the power of being your authentic self.  We strive to create the space which allows for everyone in our Adverum Community to not only feel safe but encouraged to speak, learn from each other, grow in their professions and be the very best versions of themselves no matter what their age, ethnic background, gender, origin, religion or sexual orientation