Associate Scientist, Discovery (bb)

Location
Redwood City, CA
Posted
Apr 16, 2021
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
This is a research position for a highly motivated Associate Scientist to support the Research and New Vector Technologies group. The candidate will support the early development and evaluation of AAV-based therapeutics, including vector optimization, development of in vitro cell system, and the development of research-grade assay in support of preclinical studies.
What you'll do:
  • Independently plan and execute experiments, analyze, and communicate results.
  • Proactive in solving technical problems through data analysis and troubleshooting.
  • Perform broad range of cell and molecular biology techniques (e.g. state of the art cloning methods, DNA/RNA/protein isolation, qPCR/RTqPCR, WB, ELISA, MSD assay, Immunohistochemistry, cell culture, flow cytometry, etc.)
  • Provide bioanalytical support to in vivo preclinical studies to quantify AAV vector pharmacokinetics and biodistribution.
  • Perform research-grade assays (WB, ELISA, MSD, qPCR, RTqPCR, immunohistochemistry).
  • Cloning of vector constructs and in vitro screening for expression and functionality, in cell lines and/or tissue explant cultures.
  • Provide support for the QC analysis of AAV vector lots (qPCR/ddPCR titration, WB, SS, endotoxin assay, gel analysis).
  • Support and assist the members of the team, as needed.

About you:
  • Minimum of 8 year for BS or 5 years for MS, of research experience in academia/industry
  • Strong proficiency with molecular biology technics, including but not limited to molecular cloning, site-directed mutagenesis, PCR, mRNA and DNA quantification/analysis (RT/qPCR, ddPCR), sequencing (Sanger), protein analysis and immunoassays (Western Blot, ELISA), plate-based reporter and/or enzymatic activity assays.
  • Experienced in immunohistochemistry and microscopy is a plus.
  • Strong skills with demonstrated track record in cell culture: sterile technique for handling and maintenance of mammalian/insect cells, non-viral or viral gene transfer, DNA editing and flow cytometry.
  • Previous experience with stable cell line development, tissue explant cultures, primary cell lines and/or iPS is a plus.
  • Excellent data analysis skills and experience with a variety of scientific software applications is required (including Microsoft Office, Prism, Geneious, etc).
  • Demonstrable track record of solving technical problems through data analysis and troubleshooting.
  • Excellent communication, presentation, collaboration, time-management, and organization skills are required.
  • Proven ability to work independently under minimal supervision, self-motivated to learn and develop new techniques, perform multiple tasks simultaneously, keep accurate and timely records, follow instructions, and comply with company policies.
  • Familiarity with drug product development and AAV gene therapy is a plus.
  • Work authorization in the US required.

About Us
Adverum is a clinical-stage gene therapy company targeting unmet medical needs in ophthalmology and rare diseases. Adverum develops gene therapy product candidates designed to provide durable efficacy by inducing sustained expression of a therapeutic protein. Adverum’s core capabilities include clinical development, novel vector discovery and in-house manufacturing expertise, specifically in scalable process development, assay development, and current Good Manufacturing Practices quality control.

At Adverum, Inclusion and Diversity are at our core. We believe in the power of being your authentic self.  We strive to create the space which allows for everyone in our Adverum Community to not only feel safe but encouraged to speak, learn from each other, grow in their professions and be the very best versions of themselves no matter what their age, ethnic background, gender, origin, religion or sexual orientation