Flow Core Supervisor

Location
Cambridge
Posted
Apr 03, 2021
Ref
5044366002
Required Education
Masters Degree/MBA
Position Type
Full time

JOB PURPOSE

Sana Biotechnology is looking to expand our team with motivated individuals who believe in the potential of cell and gene therapy, as well as have the vision to do creative things and the determination to execute that vision. We are looking for a Flow Core Supervisor to support the establishment of a flow cytometry core for the Development Sciences function at our Cambridge site. This individual will be responsible for routine instrument maintenance, support of study and panel design, process development, and vendor contract management under the direction of the Flow Core Manager. The Flow Core Supervisor will work with internal project team leads to support and streamline flow cytometry needs across multiple programs. This individual will lead the strategy, design, and implementation of sample inventorization and ELN data management. This individual will have strong communication skills to train new users on principles of flow cytometry, guidelines for panel design, and instrument use, and to convey adapting needs from end users to the Flow Core Manager. The Flow Core Supervisor will possess demonstrated technical proficiency, scientific creativity, collaboration skills, and independent thought to develop and enact creative solutions to optimize flow cytometry platforms across the Development Sciences function.

DUTIES AND RESPONSIBILITIES

 

  • Perform routine flow cytometer maintenance and QC (Fortessa, Symphony)
  • Manage service contracts and repairs, and serve as primary point of contact for relevant vendors
  • Provide panel design and experimentation support for flow cytometry studies
  • Train new users on flow cytometer instrumentation
  • Operate analyzers following sample preparation and gating by study leads, as needed
  • Lead development and standardization of workstreams to maximize sample throughput and to facilitate method transfer to CROs
  • Define and maintain workflow SOPs
  • Lead design and implementation of sample and data management strategy
  • Facilitate ongoing discussion with users to optimize use of platform
  • Communicate developing flow cytometry needs and bottlenecks to Flow Core Manager
  • Support development and implementation of strategies to optimize and streamline use of platform for end users
  • Assist in identifying, evaluating, and implementing new flow cytometry techniques or strategies as a technical expert
  • Comply with standard laboratory practices and company policies
  • Contribute to building a positive, team-oriented culture


QUALIFICATIONS

Basic Qualifications

 

 

  • Bachelor's degree with 5-8 years' experience or master's degree with 3-6 years' experience with flow cytometry instrumentation and study design
  • Extensive hands-on experience with full flow cytometry assay workstream (sample collection, processing, staining, instrumentation, and data analysis)
  • Proficiency with instrument troubleshooting and flow cytometry workstream optimization strategies (e.g., color panel design, antibody selection, staining optimization)
  • Experience managing vendor contracts and interactions
  • Proficiency with flow cytometry analysis software (Diva, FlowJo), as well as with MS Office (Word, Excel, PowerPoint) and basic statistical packages (e.g., GraphPad Prism)
  • Understanding of basic in vitro and in vivo study design principles
  • Excellent interpersonal, organizational, and problem-solving skills to support multiple studies and organizational efforts at different phases
  • Ability to multi-task and to work cross-functionally with team members in matrixed environments
  • Effective communication, both verbally and in writing
  • Strong organization, record-keeping, and data documentation skills


Preferred Qualifications

 

 

  • Experience with Symphony analyzer and/or sorting a major plus
  • Experience training new users to flow cytometry, including compiling reference guidelines / training materials
  • Deep knowledge of in vitro and in vivo study design principles
  • Familiarity with PK/PD modeling
  • Management of CRO relationships +/- internal legal team interactions to put study agreements in place
  • Knowledge of the design and conduct of R&D studies for the development of cell or gene therapies
  • Familiarity with non-flow-related cell and molecular biology assays and tools (e.g., qPCR, Western, ELISA/Luminex, cell culture)


WORKING CONDITIONS

This role will be largely based in a life sciences laboratory, including the use of routine personal protective equipment (lab coat, gloves, etc.) to be provided by Sana.