Associate Scientist, Protein Process Development & Manufacturing

Foster City, CA, United States
Mar 25, 2021
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Vaxcyte, Inc. (Nasdaq: PCVX) is a next-generation vaccine company seeking to improve global health by developing superior and novel vaccines designed to prevent some of the most common and deadly infectious diseases worldwide. Our exclusively licensed cell-free protein synthesis platform and our proprietary know how enable us to design and produce optimized protein carriers and antigens, the critical building blocks of vaccines, in ways that we believe conventional vaccine technologies cannot. Our pipeline includes pneumococcal conjugate vaccine, or PCV, candidates that we believe are the most broad-spectrum PCV candidates currently in development, targeting the $7 billion global pneumococcal vaccine market. Our lead vaccine candidate, VAX-24, is a preclinical, 24-valent broad-spectrum pneumococcal conjugate PCV with preclinical proof-of- concept demonstrating potential to replace the standard of care that we expect to advance into clinical trials in the second half of 2021. Our pipeline also includes VAX-XP, a PCV with an expanded breadth of coverage of at least 30 strains, including newly emerging strains responsible for invasive pneumococcal disease and antibiotic resistance; VAX-A1, a prophylactic vaccine candidate designed to prevent Group A Strep infections; and VAX-PG, a therapeutic vaccine candidate designed to slow or stop the progression of periodontal disease by targeting the keystone pathogen responsible for this chronic, oral inflammatory disease. We completed our initial public offering in June 2020, raising $287.5 million in gross proceeds.


The Development organization is comprised of four Process teams: Protein, Polysaccharide, Conjugate Drug Substance, and Drug Product. These Process Development teams are supported by Analytical Development and Formulation Development teams. Vaxcyte is looking for an Associate Scientist to join the Protein Process Development & Manufacturing team to develop the downstream purification processes for multiple protein antigens that are part of the VAX-A1 vaccine.

Essential Functions:

• Develop the downstream purification process for multiple protein antigens that comprise the VAX-A1 vaccine ; the purification process must be robust, scalable, and cost-effective
• Collaborate with Analytical Development to implement and run assays to assess product quality and purity
• Generate material for analytical method and conjugation development
• Participate in cross-functional project teams and provide regular progress updates
• Participate in technology transfer activities for the process developed at Vaxcyte to an external CMO for GMP production


• BS in Chemical Engineering, Biochemistry, or a related discipline, with 3+ years of industry experience; MS with 1+ years of industry experience
• Strong protein purification knowledge and experience, including familiarity with AKTA lab-scale and preparative chromatography systems and UNICORN software
• Experience with depth, nominal, and tangential flow filtration
• Hands-on experience purifying untagged proteins from crude lysate to high purity
• Solid understanding of the principals of DoE (Design of Experiments); practical experience with DoE software
• Both practical experience with and theoretical knowledge of engineering principles involved in scaling processes from development lab to pilot / manufacturing plant
• Working knowledge of the requirements of GMP manufacturing ; hands-on GMP experience a plus
• Experience working with CMOs highly desired; ability to travel to CMO (some international travel required) to perform person-in-plant oversight activities
• Proficiency running gel- and column-based analytical methods a plus; such methods may include SDS-PAGE, Western blotting, IEF, SEC, HIC, IEX, RP, Mass Spec, and binding affinity or activity assays (such as MSD or ELISA)
• Ability and desire to learn upstream cell-free production system used to express proteins
• Practical experience with microbial fermentation and running bioreactors / fermenters (up to 50 L scale) a plus
• Demonstrated success working in a cross-functional team environment on multiple projects; ability to work both on a team and independently to deliver results
• Strong interpersonal skills, with excellent written and verbal communication skills

Reports to: Associate Director, Protein Process Development & Manufacturing

Location: Foster City, CA


The compensation package will be competitive and includes comprehensive benefits and an equity component.