San Diego, CA, US
Mar 18, 2021
Biotech Beach
Required Education
Position Type
Full time
Those who join Emergent BioSolutions feel a sense of ownership about their future. You will excel in an environment characterized by respect, innovation and growth opportunities. Here, you will join passionate professionals who advance their scientific, technical and professional skills to develop products designed-to protect life.

Position Summary

Conduct cell line development for recombinant proteins by designing, constructing, and evaluating expression vectors. Perform vector design, construction, and characterization to generate highly productive, high quality, robust cell lines that meet all regulatory requirements. Perform single cell cloning and screen to identify high production clones. Conduct mammalian cell culture in shake flasks and small scale bioreactors to evaluate clones. Document of activities in lab notebooks and draft SOPs. Perform medium and feed optimization of selected clones.

Essential Functions

Reasonable accommodations will be made to enable individuals with disabilities to perform the essential functions.
  • Cell line development related activities including molecular cloning, transfection, selection, single cell cloning and cell banking.
  • Perform mammalian cell cultures in multi well plates, shake flasks, and small-scale bioreactors.
  • Optimize media and feed for selected clones.
  • Operate related equipment.
  • Troubleshooting the issues related to cell line development.
  • Write technical reports , SOPs and tech transfer reports
  • Use DOE software to design the experiment and analyze the results.
  • Drive improvements in recombinant proteins production by designing, constructing, and evaluating expression vectors as required.

Minimum Education, Experience and Skills
  • PhD in molecular biology and cell biology or related discipline is required. PhD degree with 1-2 years relevant industry experience. MS or BS with 5 years industry experience in cell line development.
  • Hand on experience with molecular cloning, transfeciton, mammalian cell cultrue of various adherent cells and suspention cells such as Vero, HEK293, CHO cells is required.
  • Experience with automation and high-throughput technologies as applied to mammalian cell line generation and process optimization is a plus.
  • Expereince with design of experiment (DOE) to eveluate clones in Ambr® bioreactors is a plus.
  • Experience with cell culture media analysis and optimization is a plus.
  • Experience with quantitative and qualitative methods, including ELISA, MSD, fluorescence and IF microscopy, colorimetric, enzymatic assays and reporter assays, FACS assays is prefered.
  • Experience with protein expression and detection by Western Blot and immunostaining is preferred.
  • Ability to think critically, and demonstrated troubleshooting and problem-solving skills
  • Engineering knowledge/hands on experience with equipment troubleshooting is preferred.
  • Good writing skills and communication skills.
  • Willingness to expand skill set and responsibilities as required.

There are physical/mental demands and work environment characteristics that must be met by an individual to successfully perform the essential functions of the job. This information is available upon request from the candidate.

Reasonable accommodations may be made to enable individuals with disabilities to perform all essential functions.

Emergent BioSolutions is an Equal Opportunity/Affirmative Action Employer and values the diversity of our workforce. Emergent does not discriminate on the basis of race, color, creed, religion, sex or gender (including pregnancy, childbirth, and related medical conditions), gender identity or gender expression (including transgender status), sexual orientation, age, national origin, ancestry, citizenship status, marital status, physical or mental disability, military service or veteran status, genetic information or any other characteristics protected by applicable federal, state or local law.

Information submitted will be used by Emergent BioSolutions for activities related to your prospective employment. Emergent BioSolutions respects your privacy and any use of the information submitted will be subject to the terms of our Privacy Policy .

Emergent BioSolutions does not accept non-solicited resumes or candidate submittals from search/recruiting agencies not already on Emergent BioSolutions' approved agency list. Unsolicited resumes or candidate information submitted to Emergent BioSolutions by search/recruiting agencies not already on Emergent BioSolutions' approved agency list shall become the property of Emergent BioSolutions and if the candidate is subsequently hired by Emergent BioSolutions, Emergent BioSolutions shall not owe any fee to the submitting agency.

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