Sr. Research Associate II

Location
San Diego, CA
Salary
Up to 95K w/ full benfits
Posted
Feb 19, 2021
Ref
Locana-Sr. RA
Required Education
Bachelors Degree
Position Type
Full time

BioPhase Solutions

This Sr. Research Associate II will be responsible for DNA plasmid design, synthesis and testing in cell-based assays.  We need a highly motivated individual with molecular biology and gene expression experience for development of cell-based assays, from cloning to testing, that will contribute to the identification of potential therapeutics.

Responsibilities:  

·        Responsible for the design of novel DNA constructs and cloning strategies.

·        Test constructs in cell-based assays.

·        Perform extraction of DNA, RNA, and proteins followed by testing using PCR, qRT-PCR,      Immunofluorescence, in situ hybridization, Western blotting, ELISA, and related molecular biology techniques.

·        Examine data and prepare technical reports, summaries, protocols and quantitative analysis, ensuring experiments are well documented.

·        Maintain familiarity with current scientific literature as advised by supervisor.

·        Work in a team environment, collaborating with various colleagues across multiple departments.

·        Help to maintain laboratory, order common supplies, and contribute to plasmid production for collaborating groups.

·        Perform additional activities as needed.

Requirements:

·        BS or MS in Molecular Biology, Biochemistry, Cell Biology or related field,

·        5 - 8 years experience using molecular and cell biology techniques.

·        Knowledge and experience with: molecular cloning and DNA plasmid design; mammalian tissue culture and cell based assays; nucleic acid isolation, PCR, qPCR and detection techniques is required.

·        Excellent written and oral communication skills.

·        Desire to be part of a rapidly evolving organization which requires flexibility, and a willingness to learn new techniques and methods.

Preferred Qualifications:

·        Experience or willingness to work with culturing, differentiating and/or reprogramming primary patient fibroblasts and animal-derived tissues (e.g., RNA extraction).

·        Experience with automated liquid handlers.

·        Experience with flow cytometry.

·        Familiarity with genome browsers and other sequencing manipulation software tools.