Process Engineer - Cell Therapy Process Development

Location
South San Francisco, CA, United States
Posted
Jan 22, 2021
Ref
5043864002
Required Education
Bachelors Degree
Position Type
Full time
JOB PURPOSE

Sana Biotechnology has an exciting opportunity for a Process Engineer within the Cell Therapy Process Development group. The Process Engineer will focus on the development of a novel chimeric antigen receptor T (CAR T) cell manufacturing process. Under the supervision of the process development lead, the candidate will contribute to the design of multiple unit operations including cell selection and expansion, gene editing, and cryopreservation. The Process Engineer is expected to closely collaborate with multiple teams including Analytical Development.

DUTIES AND RESPONSIBILITIES
  • Design and perform small- and large-scale cell therapy experiments using primary cells across multiple unit operations including cell selection, expansion, gene editing, cell harvest and cryopreservation
  • Perform in-process analysis of samples including cell counting and metabolite analysis
  • Analyze data, present conclusions and process design recommendations to a multidisciplinary group
  • Document all experimental work in an electronic laboratory notebook
  • Draft technical documents including reports, operating procedures, master batch records and bioreactor recipes,

QUALIFICATIONS

Basic Qualifications
  • BSc. in Chemical, Biological or Biomedical Engineering plus 4 yrs or MSc plus 1-2 yrs or equivalent combination of education and work experience
  • >2 year of experience with mammalian cell culture
  • Excellent aseptic cell culture technique and good scientific method
  • Detail-oriented with good problem-solving, technical writing and verbal communication skills
  • Ability to effectively work in multidisciplinary teams, meet deadlines, and prioritize multiple projects
  • Aptitude for working in a self-driven, performance/results-oriented, fast-paced matrix environment
  • Ability to engage in crucial conversations - providing and receiving feedback
  • The candidate should be a team player, highly organized with excellent communication skills, demonstrate curiosity and scientific rigor and excellent problem-solving skills

Preferred Qualifications
  • Working knowledge of statistical Design of Experiments (DoE)
  • Prior experience participating in the design of a clinical cell therapy manufacturing process
  • Knowledge of immunology with focus on T cell biology
  • Experience with cell therapy unit operations including:
    • Cell selection
    • T cell culture in bioreactors
    • Viral and non-viral gene editing methods
    • Cell washing and harvesting
    • Cryopreservation
  • Operating knowledge of small to mid-scale bioreactors operations (ambr 15, stirred tank and rocking motion) including drafting recipes in MFCS
  • Experience with analytical methods including:
    • Cell counts and viability using automated cell counters
    • Media metabolite analysis
    • Flow cytometry
    • Cell-based assays, including Incucyte systems
  • Prior experience supporting tech transfers to external partners
  • Demonstrate intense technical curiosity and a continuous drive to learn new skills
  • Knowledge of R, Python, JMP, SpotFire, Benchling and FlowJo software

WORKING CONDITIONS
  • This position requires handling of human blood products
  • Some weekend work required
  • Work in a laboratory environment approximately 75% of the time

PHYSICAL REQUIREMENTS
  • Able to lift up to 25 lb.