Scientist I, Gene Circuit Discovery

Location
South San Francisco, California, United States
Posted
Jan 07, 2021
Ref
729087448
Hotbed
Biotech Bay
Required Education
Doctorate/PHD/MD
Position Type
Full time
Thank you for your interest in a career at Senti Biosciences. We are passionate about developing cell therapies with the ability to outsmart cancer and other complex diseases. We find purpose and meaning in knowing that our efforts will soon improve the lives of patients and their families. With science as our foundation and empathy as our key guiding principle, colleagues become friends, managers become mentors, and with time, cancer becomes history.

Senti Bio is seeking a collaborative and passionate Scientist to join our Gene Circuit Discovery group. This is an individual contributor role that will apply expertise in synthetic biology to design and test gene circuits for the generation of regulated cell and gene therapies in primary immune cells (e.g. NK cells, T cells, macrophages, and/or monocytes). The ideal candidate will be comfortable planning and independently executing in vitro experiments in immune cells, communicating findings, and collaborating with Senti colleagues to push projects forward in a fast-paced startup environment. Additionally, the successful candidate will value and demonstrate Senti's core employee attributes: empathetic, collaborative, intellectually curious, and intrinsically motivated.

Responsibilities
    • Design and optimize therapeutic genetic circuits composed of parts including engineered proteins, synthetic promoters, and RNA-based regulatory elements
    • Generate viral vectors for gene circuit transduction into multiple mammalian cell types including primary immune cells
    • Evaluate gene circuit performance using a variety of in vitro assays including multi-color flow cytometry, microscopy, ELISA, and Luminex
    • Analyze data and present results to downstream stakeholders
    • Document experiments by generating timely records of protocols and data using Senti's electronic lab notebook
    • Collaborate with your colleagues at Senti by coordinating with other group members and research teams to troubleshoot problems, develop new assays, and support cross-functional projects
    • Stay current with the relevant scientific literature, and participate in ideation and decision-making meetings
    • Provide expert support for experiment design and assay development to the broader scientific team


Qualifications
    • Ph.D. or equivalent with expertise in synthetic biology, immunoengineering, bioengineering, biotechnology, or cell engineering
    • Proficient with mammalian cell culture and mammalian cell engineering, preferably for therapeutic applications
    • Capable of designing and implementing multicolor flow cytometry experiments
    • Desire to be a team player in a fast-paced environment
    • Comfortable presenting work to cross-functional groups


Preferred Qualifications
    • Proficient in primary immune cell culture and engineering by lentiviral or other methods (e.g. NK cells, T cells, macrophages, and/or monocytes)
    • Experience with assaying immune cell states and behaviors, including cell surface markers, cytokines, migration, and cytotoxicity
    • Experience with NGS sample prep and analysis
    • Molecular understanding of transcriptional regulation
    • Familiarity with FlowJo, SnapGene, (Prism and Benchling are also a plus)
    • Experience in mammalian synthetic biology is a plus, such as design of synthetic transcriptional and/or translational regulation to control therapeutic gene expression


Salary & Benefits
    • Base pay, bonuses, and benefits to be determined based on application qualifications with the aim of being competitive with similar roles in the area
    • Significant growth opportunity as the company expands


Senti Bio is the next generation therapeutics company that designs gene circuits and programs cells for tremendous therapeutic value. Our mission is to engineer an entirely new class of medicine that will transform people's lives by curing the most challenging diseases. We are proud to count NEA, 8VC, Amgen Ventures, Pear Ventures, Lux Capital, Menlo Ventures, Allen & Company, Nest.Bio , Omega Funds, Goodman Capital, and LifeForce Capital among our investors. Senti Bio is based in South San Francisco, CA, USA.