Principal Scientist, Protein Sciences

San Diego, California, United States
Dec 21, 2020
Biotech Beach
Required Education
Position Type
Full time
About Prometheus Biosciences, Inc.

Prometheus Biosciences, Inc. is a privately held biotechnology company pioneering a precision medicine approach to the discovery, development, and commercialization of novel therapeutic and companion diagnostic products for the treatment and diagnosis of IBD. The company's precision medicine platform, Prometheus 360, combines proprietary bioinformatics discovery methods with one of the world's largest gastrointestinal bioinformatics databases to identify novel therapeutic targets and develop therapeutic candidates to engage those targets. Prometheus is guided by its board of directors, led by Chairman Tachi Yamada, M.D., and a scientific advisory board composed of key opinion leaders in IBD, including Stephan Targan, M.D., William Sandborn, M.D. and Dermot P. McGovern, M.D., Ph.D. In addition, Prometheus has entered into collaborations to develop targeted therapies for IBD.

The Principal Scientist, Protein Sciences will have a deep and broad knowledge of protein analytical technology, including chromatographic and biophysical characterization methods. He/She will lead efforts to assess the developability of monoclonal antibodies and communicate the strategies and results to both the team and company.

ESSENTIAL DUTIES AND RESPONSIBILITIES include the following. Other duties may be assigned.
  • Manage purification, analytical characterization and developability assessment of monoclonal antibodies and other biologics modalities to support candidate selection and pre-clinical development of therapeutic programs.
  • Lead analytical characterization of reagents and engineered constructs.
  • Present and communicate strategies, concepts and results to multidisciplinary drug discovery teams.
  • Lead and mentor scientists; facilitating their contributions to the team, the company and career development. Contribute to a culture that values continuous learning and development.
  • Stay abreast of latest scientific and competitive developments around therapeutic platform choice/s and experimental methods; including solubility, aggregation, glycol- and charge-variant analysis, stress testing and peptide mapping of antibodies and establishing working relationships with CROs.

May supervise employees within the Research Department either directly or via matrix management. Carry out supervisory responsibilities in accordance with the organization's policies and applicable laws. Responsibilities include leading and mentoring scientist, facilitating their contributions to team, the company their career development.

  • Deep and broad knowledge of protein analytical technology including chromatographic and biophysical characterization methods such as ultrahigh-pressure liquid chromatography (UPLC) and high performance liquid chromatography (HPLC) (size-exclusion chromatography, ion-exchange, reversed- phase, hydrophobic chromatography), capillary electrophoresis, capillary isoelectric focusing, dynamic and static light scattering (DLS, MALS), differential scanning calorimetry (DSC), differential scanning fluorimetry (DSF), viscosity and stability assessments.
  • Excellent organization in day to day experiments and timely updating of experiment files
  • Supervisory experience.
  • Preferred - Characterization of antibody/protein kinetics by e.g. SPR/BLI.

  • Ph.D. in protein chemistry, biology, biochemistry or related discipline with at least 8 years of experience in protein analytical characterization or BS/MS degree with at least 12 years of experience in protein purification and analytical characterization.

  • Ability to effectively communicate cross-functionally with team members throughout the organization.
  • Ability to demonstrate communication effectively (both written and oral)
  • Strong ability work with collaboration externally and internal matrixed teams
  • Ability to drive complex programs



The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. While performing the duties of this job, the employee is regularly required to sit and talk or hear. The employee frequently is required to use hands to finger, handle, or feel. The employee is occasionally required to stand, walk, and reach with hands and arms. The employee must occasionally lift and/or move up to 25 pounds. Specific vision abilities required by this job include close vision, distance vision, peripheral vision, depth perception, and ability to adjust focus.

The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. While performing the duties of this job, the employee is occasionally exposed to moving mechanical parts, fumes or airborne particles, potentially infectious bodily fluids, toxic or caustic chemicals, and extreme cold. The noise level in the work environment is moderate.

The noise level in the work environment is usually quiet.

All qualified applicants are considered for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity, national origin, disability, veteran status or other protected class.