AbbVie

Scientist II - Assay Development

Employer
AbbVie
Location
Sunnyvale, CA, United States
Posted
Nov 10, 2020
Ref
2008381
Required Education
Bachelors Degree
Position Type
Full time
About AbbVie
AbbVie's mission is to discover and deliver innovative medicines that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas: immunology, oncology, neuroscience, eye care, virology, women's health and gastroenterology, in addition to products and services across its Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com . Follow @abbvie on Twitter , Facebook , Instagram , YouTube and LinkedIn .

General Position Summary/Purpose:

The Scientist II will be a key member of the Discovery Biology team responsible for providing strong technical expertise and implementation to support drug discovery with special emphasis on assay development, compound screening and automation. The Scientist II will be responsible for the development and hands on execution of various biochemical/biophysical and cellular assays suitable for compound screening in HTS/automation mode to support small-molecule drug discovery projects. The successful candidate will be expected to conceive and execute novel scientific approaches to achieve departmental project goals and clearly communicate the scientific rationale, methodology and results in written and oral formats.

Key Accountabilities/Core Job Responsibilities:
  • Demonstrate scientific knowledge and independence to establish and implement strategies for exploring the mechanisms of action of novel molecules to support translation into future therapies for new indications in hematological malignancies.
  • Hands-on execution: Advance science from the bench, design and execute various biochemical, biophysical and cellular assays including whole blood assays in HTS format to determine potency and selectivity of small molecules to understand their SAR and mechanism of action.
  • Work with supervisors to improve assay protocols and take the leading role in the development, optimization, and validation of new assays. Utilize technical expertise and available information to troubleshoot and resolve key assay hurdles when encountered.
  • Accurately document, critically analyze/interpret data and communicate the results promptly.
  • Actively participate in scientific meetings by presenting and critically evaluating emerging scientific literature to facilitate further progress and success of key project assignments.
  • Perform tasks with diligent attention to detail and high level of accuracy. Prioritize tasks and contribute to a highly motivated and productive research team with a sense of urgency and desire to meet project timelines.

Qualifications

Qualifications:
  • BS with 5 or more years of relevant laboratory experience or MS with 3 or more years of relevant laboratory experience either in academic or industrial settings.
  • Familiarity with techniques and instrumentation relevant to automated plate-based assays and the use of multiple techniques to execute the desired assays in an HTS format.
  • Demonstrated ability to design, execute and interpret experiments via publications, external presentations or patents.
  • Strong interpersonal skills to thrive in a team environment.
  • Effective written and verbal communication skills.


Experience:
  • Extensive experience in develop assays using purified-components and cell-based systems configured to identify small-molecule inhibitors or activators and their automation to enable high-throughput via Liquid handlers is a must.
  • Experience in designing and executing biochemical/biophysical microplate-based assays to identify and characterize small-molecule modulators using a variety of techniques and detection methods, including FP, FRET, TR-FRET, BRET, SPR, MST, CETSA, MSD, Lab-Chip MSA, mass spectrometric, chromogenic, and radiometric formats.
  • Participation in project teams including Chemistry, Bioassays and HTS colleagues deploying biochemical/biophysical and cellular assays including whole blood assays for high-throughput screening as well as to generate dose response curves to drive SAR development.
  • Basic knowledge of protein chemistry including protein expression, purification, labelling and characterization by various biotechniques such as Protein Simple WES system is highly desirable.
  • Experience in establishing and fully validating compound potency assays using cell lines or primary human cells is essential. Experience in using cell biology models to elucidate molecular mechanisms of action.
  • Good knowledge of programing, designing and executing various biochemical, biophysical and cellular assays using Automated Liquid Handlers is highly desirable.
  • Basic knowledge on biological data analysis tools (GraphPad Prism and Microsoft Office tools).
  • Contribute to lab and equipment maintenance and help create a safe, effective and efficient working environment.

Significant Work Activities
Awkward/forceful/repetitive motions (arms above shoulders, bent wrists, etc.)
Travel
Yes, 5 % of the Time
Job Type
Experienced
Schedule
Full-time
Job Level Code
IC
Equal Employment Opportunity
At AbbVie, we value bringing together individuals from diverse backgrounds to develop new and innovative solutions for patients. As an equal opportunity employer we do not discriminate on the basis of race, color, religion, national origin, age, sex (including pregnancy), physical or mental disability, medical condition, genetic information gender identity or expression, sexual orientation, marital status, protected veteran status, or any other legally protected characteristic.