Scientist, Cell & Gene Therapy Technologies
- Employer
- Orchard Therapeutics
- Location
- London, United Kingdom
- Start date
- Oct 14, 2020
View more
- Discipline
- Science/R&D, Biotechnology, Genetics
- Required Education
- Doctorate/PHD/MD
- Position Type
- Full time
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Orchard Therapeutics, Ltd. (OTL) is looking for an experienced Scientist to develop and optimize lentiviral vector (LVVs) platform(s). This is a great opportunity to join the growing Process Development team within Cell & Gene Therapy Technologies department and contribute to the delivery of Orchard Therapeutics innovative gene-modified cell-based therapeutic products. The job role will include molecular biology, cell line development, process development, process characterization and manufacture of lentiviral vectors. The post-holder will also input into the transfer and implementation of optimized processes to GMP to support OTL's product pipeline programs.
Key Elements and Responsibilities
Requirements
Required knowledge
Skills & Abilities
Education
Key Elements and Responsibilities
- Develop stable cell lines for LVVs;
- Design, develop and improve process(es) (USP and DSP) for LVVs to deliver robust manufacturing platforms for early and late-stage clinical and commercial development;
- Develop and perform cell-based and biochemical assays to characterise LVV processes and products;
- Execute experimental plans, analyse and interpret experimental data, maintain experimental results in relevant databases and write technical reports;
- Provide hands-on technical expertise/scientific guidance to team members and supervision of junior colleagues;
- Contribute to the routine lab maintenance;
- Contribute to the transfer of stable cell lines and improved processes to GMP and assist with oversight of manufacturing activities as required;
- Prepare, review and complete protocols/SOPs, study reports and assist with regulatory submissions;
- Communicate, share and discuss work progress internally and externally;
- Other responsibilities may be assigned.
Requirements
Required knowledge
- In-depth knowledge on the state-of-the-art of cell & gene therapy products and available technology, more specifically regarding viral vectors;
- Minimum of PhD+3 years (academic/industrial) or minimum of 7 years (industrial) of experience with development, manufacture and delivery of viral vectors, preferably retro and lentiviral vectors;
- Demonstrated skills and hands-on experience in tissue culture techniques and handling of adherent and suspension cell lines in different culture systems;
- Demonstrated skills and hands-on experience in molecular biology and vectorology, including cloning, standard DNA and RNA technology, analyses with qPCR, recombinant protein expression and assessments with SDS-PAGE, Western blot, ELISA, flow cytometry and others;
- Demonstrated knowledge and hands-on experience with stable cell lines development for retro and lentiviral vectors;
- Demonstrated knowledge in designing and developing processes for LVV production and purification;
- Experience in developing and performing analytical methods for stable cell lines and LVV characterization;
- Use of more specialist lab equipment e.g. flow cytometers, cell counters, cell sorters, microscopes and imaging systems, bioreactors, AKTA, TFF systems, controlled-rate freezers etc;
- Proficient with MS office, Prism, SnapGene, FlowJo, e-lab books and other commonly used software;
- Experience with DoE software (s) and statistical analyses is a plus;
- Understanding of and familiarity with regulatory guidance governing stable cell lines and vector-based products is a plus;
Skills & Abilities
- Ability to work collaboratively as part of a multi-disciplinary team, or independently as necessary;
- to prioritise work and meet deadlines;
- Independently design and execute experiments following laboratory procedures, appropriately analyse data and provide an interpretation of results;
- and written skills for report writing, team communications and presentations;
- Creativeness, motivation, and troubleshooting skills to solve problems and/or develop new technical capabilities;
- Ability to operate in a fast paced, multidisciplinary industrial environment
Education
- A minimum PhD or equivalent in biotechnology, process development, cell line development, cell and gene therapy, biological sciences, or other relevant scientific discipline.
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