Scientist, Cell Engineering

Location
Philadelphia, PA, United States
Posted
Oct 13, 2020
Ref
1938590911
Required Education
Doctorate/PHD/MD
Position Type
Full time
Century Therapeutics is developing novel allogeneic living cell products for oncology that overcome the limitations of current cell therapies. Century's genetically-engineered, universal iPSCs-derived immune effector cell products are designed to specifically target hematologic and solid tumor cancers. Our commitment to developing off-the-shelf cell therapies will expand patient access and provide an unparalleled opportunity to advance the course of cancer care.

Century's foundational technology is built on induced pluripotent stem cells (iPSCs) that have unlimited self-renewing capacity. This enables multiple rounds of cellular engineering to produce master cell banks of modified cells that can be expanded and differentiated into immune effector cells to supply vast amounts of allogeneic, homogeneous therapeutic products. This platform differentiates Century from other approaches that utilize non-renewable donor-derived cells.

Role:

We are seeking a highly motivated and creative scientist with expertise in the cell engineering and gene editing space, to help us advance our universal iPSC-derived allogeneic cell products for targeting hematological and solid tumors. The Scientist / Senior Scientist, Cell Engineering will be responsible for utilizing existing cell engineering methodologies, as well as developing and incorporating novel tools for targeted gene editing in iPSCs in support of cell therapy efforts. The ideal candidate will work closely with research colleagues and collaborate with external partners and functional teams to achieve our collective corporate goals.

Qualifications:

PhD in biology or related discipline; a strong research track record in either Biotech/Pharma or academia with more than 2-3 years of relevant experience
  • Proven expertise in genome editing techniques including CRISPR-Cas9, Cpf1, and Base Editing
  • Demonstrated experience with targeted gene insertion into the genome by Homology-Directed Repair.
  • Extensive experience in cell and molecular biology, including molecular cloning, lentivirus transduction, gene- and protein expression analysis
  • Ability to perform in a fast-paced environment and handle multiple priorities simultaneously


Responsibilities:
  • Gene editing at target gene loci using CRISPR-Cas9 and Cas12a in iPSCs
  • Targeted gene insertions using Homology Directed Repair (HDR) and optimizing gene knock-in, knock-out (KIKO) systems in iPSCs
  • Application of dsDNA break-free genome editing including base editing and rAAV-mediated HDR
  • Working closely with the Bioinformatics group to characterize off-target effects post-gene editing using GUIDE-seq, CIRCLE-seq or other relevant approaches
  • Cloning constructs into expression vectors
  • Possess the ability to independently propose, design, execute and report research projects


Requirements:
  • Experience in genome editing using DNA base editing is highly desirable
  • Extensive cell culture experience essential; experience with culturing iPSCs preferred but not essential.
  • Experience with multi-color flow cytometry essential. Cell sorting experience is a plus.
  • Proficiency in molecular biology techniques including molecular cloning, qPCR and Western Blotting.
  • Strategic thinker with respect to positioning and clinical differentiation of pipeline compounds in oncology arena; appreciates the competitive oncology landscape
  • Decisive, proactive and organized with a keen ability to prioritize
  • High scientific integrity, committed to ethics and scientific rigor
  • Excellent verbal and written communication skills with the demonstrated ability to make effective presentations to a broad range of audiences, both internal and external

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