Research Associate - Marson Lab

Location
San Francisco, CA, United States
Posted
Oct 04, 2020
Ref
REQ-523
Hotbed
Biotech Bay
Required Education
Associate Degree
Position Type
Full time
Category:
Science
Lab/Area:

Marson Lab

Description:

We are recruiting a Research Associate (RA) to assist with ongoing multidisciplinary cellular genome engineering projects. Our lab has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells (Schumann et al., PNAS 2015). These new technical abilities have furthered our labs research into the genetic underpinnings of autoimmune disease and T cell biology (Farh and Marson et al., Nature 2015) and opened the door to novel cancer immunotherapies based on engineered human immune cells (Roth et al., Nature 2018). In addition to cell editing, our lab integrates genomic analysis (e.g. ChIP-seq, ATAC-seq, RNA-seq), human disease genetics, and high dimensional screening approaches for in vitro and in vivo studies of T cell function (Simeonov et al. Nature 2017; Shifrut and Carnevale et al. Cell 2018; Roth et al. Cell 2020).

Working as part of a multidisciplinary group, the RA will use CRISPR-Cas technologies to modulate gene expression for the discovery of novel coding and non-coding gene functions in immune cells for cancer immunotherapies. The primary responsibilities of the RA will be to generate the molecular components, such as DNA constructs, plasmids and lentiviral vectors for cellular engineering experiments. Additional major responsibilities will consist of isolation and culture of primary human immune cells, as well as functional analysis of engineered cells via high throughput flow cytometry. Further responsibilities will include the preparation, sequencing, and analysis of next-generation sequencing libraries to support genomic experiments. Strong organizational and communication skills are required. For exceptionally skilled applicants, opportunity exists to extend the RA's role to include the design, completion, and analysis of cellular editing experiments, as well as in vivo analysis of novel cellular cancer immunotherapies. This position represents an excellent opportunity to gain exposure to basic laboratory skills as well as genetics and genome engineering skills in a dynamic, well-established research environment.

Payment is according to the Gladstone Institutes pay scale. The J David Gladstone Institutes is an equal opportunity/affirmative action employer. All qualified candidates are encouraged to apply.

Qualifications, Licenses and Certifications

Required Qualifications:

  • BA/BS degree and one or more years of laboratory experience utilizing techniques or methods required by the position; or an equivalent combination of education and experience
  • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.)
  • Excellent organizational and interpersonal communication skills (verbal and written)
  • Willingness and ability to learn new methods and skills for changing research priorities
  • Ability to work independently and as a member of a research team
  • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines


Preferred Qualifications:

  • Prior experience with culturing human cells
  • Prior experience in the design and assembly of DNA constructs (via restriction digestion and ligation, Gibson assemblies, Golden-Gate assemblies, etc.)
  • Prior experience with next-generation sequencing library preparations and quality control
  • Prior experience with flow cytometry



Additional Information:

  • Title and salary will be commensurate with education and experience.
  • Be a part of big ideas and consider Gladstone. Your difference will make a difference in human health.
  • Gladstone is an equal opportunity employer.