Research Associate II/III, AAV

Location
Durham, NC, United States
Posted
Sep 28, 2020
Ref
1356726
Discipline
Science/R&D, Research
Hotbed
Bio NC
Required Education
Bachelors Degree
Position Type
Full time
The Research Associate assists Adeno-Associate Virus (AAV) production and purification by running tasks that range from cell culture maintenance and transfection to purification and titer determination. This role will also be expected to help with general lab upkeep and may be asked to assist with large scale DNA preparation and protein expression/purification depending on demand.

Essential Duties and Responsibilities

Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions of this position. This list contains the major duties and requirements of the job and is not all-inclusive. Incumbent(s) may be expected to perform role-related duties other than those contained in this document.
  • AAV preparation
    • Cell culture
    • Transfection
    • AAV purification
    • Titer determination
  • Cloning
    • Kinase/anneal oligos
    • Ligation
    • Transformation of E. Coli
    • Bacterial culture
    • DNA purification
    • PCR/Restriction digest
    • Agarose gel electrophoresis
    • Sequence analysis
  • Protein expression and purification
  • DNA maxi / giga preps and other general molecular biology tasks
  • Monitor the inventory for cell culture lab, and order/stock reagents and consumables as necessary


Qualifications

The requirements listed below are representative of the knowledge, skill, and/or ability required to perform this job successfully.

Required:
  • Bachelors degree in Molecular Biology or related field with 2+ years of relevant experience, or an MS in Molecular Biology or related field, or equivalent combination of training and experience
  • Experience with mammalian cell culture and cloning

Preferred:
  • Prior experience with AAV production
  • Experience with sequence analysis software (Sequencher, SnapGene, etc.)

Travel Requirements
  • No travel is expected for this position.

Location
  • This is a lab-based position located at the main headquarters in downtown Durham, NC.


Precision BioSciences is dedicated to improving life through its proprietary genome editing platform, ARCUS. Precision BioSciences leverages ARCUS in the development of its product candidates which are designed to treat human diseases and create healthy and sustainable food and agriculture solutions. Precision BioSciences is actively developing product candidates in three innovative areas: allogeneic CAR T immunotherapy, in vivo gene correction, and food.

Precision BioSciences provides equal employment opportunities (EEO) to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, gender identity or expression, national origin, age, disability, genetic information, marital status, amnesty, or status as a covered veteran in accordance with applicable federal, state and local laws. Precision BioSciences complies with applicable state and local laws governing non-discrimination in employment in every location in which the company has facilities. This policy applies to all terms and conditions of employment, including, but not limited to, hiring, placement, promotion, termination, layoff, recall, transfer, leaves of absence, compensation, and training.