Protein Purification and Characterization - Associate Research Scientist

Location
San Diego, CA, United States
Posted
Sep 17, 2020
Ref
R1528855
Required Education
Bachelors Degree
Position Type
Full time
At Bristol Myers Squibb, we are inspired by a single vision - transforming patients' lives through science.In oncology, hematology, immunology and cardiovascular disease - and one of the most diverse and promising pipelines in the industry - each of our passionate colleagues contribute to innovations that drive meaningful change. We bring a human touch to every treatment we pioneer. Join us and make a difference.

Bristol Myers Squibb in San Diego is seeking a motivated individual with experience in protein purification and biochemical assays to join the Small Molecule Structural Biology and Proteomics West team in San Diego. The successful candidate will work with our team to purify proteins of interest, assess their stability and aggregation state, and test them in various biochemical and structural studies. The ideal candidate will be proficient in the purification of highly pure recombinant proteins for use in structural biology from E. coli and baculovirus expression systems, and have expertise characterizing proteins in biochemical and biophysical assays. BMS is a world leader in discovering novel therapeutics that harness the ubiquitin-proteasome system, and the successful candidate will have the opportunity to learn about this innovative drug discovery space, working with cross-functional project teams to enable novel drug discovery. Recent publications from our group include:

https://www.nature.com/articles/s41589-018-0129-x

https://www.nature.com/articles/nature18611

https://www.nature.com/articles/s41594-020-0405-9.pdf

Job Responsibilities
  • Manage or perform the expression of proteins of interest in various cell types including E. coli and insect cells.
  • Purify protein targets of interest for assays and structural studies.
  • Characterize purified proteins by various biochemical, biophysical, and structural approaches.


Qualifications
  • B.S. or M.S. in biology/chemistry/biochemistry with 2+ years of pharmaceutical industrial experience in protein purification, structural biology, or drug discovery.
  • Substantial experience with chromatographic techniques, including affinity purification using His, GST, MBP, or Avi tags, as well as ion exchange and size exclusion chromatography.
  • Knowledge of common methods for protein characterization including SDS-PAGE, western blotting, and spectrophotometry is required.
  • Experience with AKTA FPLCs is highly desirable, and experience in the production of highly pure proteins for use in structural biology is preferred. Previous experience characterizing protein samples by LC-MS would be advantageous.
  • Experience with biophysical characterization, protein crystallization, or cryoEM techniques would be advantageous but are not required.
  • Applicants must have strong organizational skills and the ability to work on multiple projects concurrently in a team-oriented environment.
  • Strong written and oral communication skills are necessary to report on and deliver scientific presentations.
  • Proficiency with Microsoft Excel, PowerPoint and Word is required.


Around the world, we are passionate about making an impact on the lives of patients with serious diseases. Empowered to apply our individual talents and diverse perspectives in an inclusive culture, our shared values of passion, innovation, urgency, accountability, inclusion and integrity bring out the highest potential of each of our colleagues.

Bristol Myers Squibb recognizes the importance of balance and flexibility in our work environment. We offer a wide variety of competitive benefits, services and programs that provide our employees with the resources to pursue their goals, both at work and in their personal lives.