Senior Research Assistant

Employer
Abalone Bio
Location
Emeryville, CA
Posted
Sep 14, 2020
Ref
AB-SRA09142020
Required Education
Bachelors Degree
Position Type
Full time

We are looking for a Senior Research Assistant with post-graduate, full-time experience in biotech (1 year minimum). The SRA will optimize and use Abalone Bio’s yeast-based antibody discovery technology platform to find antibody drug candidates, and work to optimize and characterize antibodies with in vitro and in vivo assays.

We are looking for someone who:

  • Thrives in a dynamic and demanding startup atmosphere
  • Loves handling multiple projects
  • Manages time and prioritizes well
  • Keeps careful records
  • Is enthusiastic and self-driven
  • Relates well and productively with others

Someone aligned with our core values:

  • Empathy                            Listen actively, assume goodness
  • Collaboration                     Solve problems together
  • Honesty                             Be sincere and open
  • Kindness                           Be generous and considerate
  • Gratefulness                      Value the opportunity to do good
  • Continuous improvement                     Become a little better every day

What do we offer?

 An exciting science environment, led by accomplished scientist founders. An ambitious project that delivers a new class of antibody drugs with enormous potential. A supportive first-rate research team.

Competitive compensation and benefits. 

Salary commensurate with qualifications, and stock options.

Health, dental and vision coverage, medical and parental leave, vacation and a retirement plan.

 Location

Our facilities are in the East Bay of the San Francisco Bay Area (currently in Richmond, moving to Emeryville Fall 2020).

 Environment

The Research Associate will join a 6-8 person cross-functional R&D team.

At least weekly, the full team discusses experimental results, future plans and priorities.

Managers regularly hold one-on-one meetings to hear every team member’s concerns and thoughts, discuss and set clear expectations, and provide constructive feedback.

Qualifications/Experience

 Required:

  • BS or BA degree.
  • At least 1 year of post-graduate, full-time biotech industry experience.
  • 3 or more years of hands-on bench work experience.
  • Strong background in molecular biology and recombinant DNA technology, including extensive hands on experience and skills with DNA software.

Areas of expertise:

In addition to the above requirements, candidates must have substantial first-hand experience in at least two of these areas:

  • Microbial strain DNA engineering, including generation of DNA constructs by recombination, Gibson assembly, CRISPR and genomic homologous recombination.
  • Antibody discovery and or engineering, especially use of phage or yeast surface display; antibody library design and construction and design, production and purification of antibody variants.
  • Data analysis using R, Python or similar shell-scripting computational tools.

Additional experience in several of the following is strongly preferred: 

  • Recombinant protein purification and analysis
  • Mammalian cell culture and engineering.
  • Yeast and mammalian cell-based assays, microscopy, and flow cytometry
  • Biochemical assays (e.g. ELISAs and other plate-based assays)
  • Bioinformatics, NGS sequencing and analysis.

Job Description And Specific Responsibilities: 

Core activities:

  • Incorporate drug target receptors into yeast-based discovery platform and optimize for antibody discovery
  • Perform yeast cell-based selections for functional antibodies
  • Characterize hits from selections using yeast-based assays

Related activities:

  • Design, construct, and characterize antibody libraries in yeast and E. coli
  • Produce recombinant antibodies in E. coli, yeast and/or mammalian cells
  • Make mammalian cell lines and implement mammalian cell-based assays (ERK, cAMP, IP3, internalization)
  • Generate and analyze NGS data for identification of functional antibody hits
  • Contribute to lab operation