Scientist, Antibody and Protein Expression

Location
San Diego, CA, United States
Posted
Sep 13, 2020
Ref
R1528413
Required Education
Doctorate/PHD/MD
Position Type
Full time
At Bristol Myers Squibb, we are inspired by a single vision - transforming patients' lives through science.In oncology, hematology, immunology and cardiovascular disease - and one of the most diverse and promising pipelines in the industry - each of our passionate colleagues contribute to innovations that drive meaningful change. We bring a human touch to every treatment we pioneer. Join us and make a difference.

The Discovery Biotherapeutics team at Bristol Myers Squibb seeks to understand the structure and function of complex molecules such as antibodies and endogenous proteins in order to optimize their utility as therapeutic agents. We collaborate with biology and translational scientists imbedded in the Thematic Research Centers in order to prosecute Targets and Biological pathways with disease modifying potential. By using a blend of internal and external technologies and capabilities, we work to discover and deliver first-in-class as well as best-in-class molecules that warrant clinical evaluation for patient benefit.

Bristol Myers Squibb is seeking a highly motivated and innovative scientist with demonstrated expertise in protein sciences including expression, process optimization and automation to join our Discovery Biotherapeutics team. Responsibilities will include a range of activities related to antibody and protein expression and cell line development; and the ideal candidate will be proficient in the design, optimization, and execution of these activities related to transient and stable protein expression.

The successful candidate will:
  • Execute multiple and concurrent antibody and protein expression projects to enable downstream R&D activities. Productions will utilize several platforms, including transient Expi293, ExpiCHO, and a focus on stable CHO expression.
  • Possess a detailed understanding of recombinant standard and alternative (bi-/multi-specific) antibody production methods and expression optimization.
  • Identify and execute process improvements which may include, but are not limited to, cell culture optimization, cell line engineering, vectorology, automation, DOE, and scale-down/bench-scale bioreactors.
  • Demonstrate a track record of initiative, creativity, organization and independent performance at a high level combined with attention to detail and adherence to timelines/deliverables, and documentation.
  • Participate on project teams to discover, optimize, and manufacture antibody-based biologics, and should be highly effective in collaborating with internal and external team members and CRO's.
  • Experience in external partnership management, due diligence for technology evaluation, project leadership and matrix management is a plus.


Responsibilities:
  • Protein expression of biotherapeutic candidates to support research and early development activities, including antigens, Fc-fusion proteins, standard and bi-/multi-specific antibodies.
  • Process optimization of platform expression technologies.
  • Titer assessment, metabolite monitoring and analysis, and early product quality assessment/characterization.
  • Stable CHO cell line development using GS selection systems.
  • Manage and catalogue reagent inventories and update affiliated databases, sequence curation/QC.
  • Optimize and conduct biochemical assays that may include CE-SDS, ELISA, Octet, HPLC, automated purification, and other analytics as relevant.
  • Micro/bench-scale bioreactor operations and process development.
  • Maintain current knowledge of biologics expression technologies and relevant literature.
  • Prepare/present reports, data and updates to project and functional teams.
  • Lab/equipment organization and maintenance.
  • Recognize risks and propose contingency plans.


Requirements:
  • Ph.D. in Bioengineering, Cell Biology, Molecular Biology, or related discipline plus 2 years of work experience, or 8 years of relevant work experience with an MS or 10 years with a BSc.
  • Extensive hands-on experience with aseptic technique, cell culture, and transient and stable recombinant protein production and recombinant cell line development. CHO experience preferred.
  • Antibody/protein analysis and characterization including, but not limited to ELISA/ Octet, CE-SDS, AnSEC, protein purification, etc.
  • Exceptional organizational skills and attention to detail.
  • Strong written and oral communication skills are essential.
  • Excellent time management skills and multi-tasking capabilities.
  • Excellent organizational skills.


Skills/Knowledge in one or more of the following areas are a plus:
  • Bioreactor operations (AmbrĀ®15 microbioreactors, Wave bag, bench-scale single-use and glass).
  • Automated liquid handling.
  • Standard molecular biology methods, cloning, plasmid preparation, vector design, PCR, mutagenesis, and DNA sequence analysis.
  • Plate based imaging, Flow cytometry/microscopy.
  • Vector, cell line, and expression engineering and experimental design.
  • Sample inventory management.
  • Experience and knowledge of protein and antibody structure, sequences, engineering, display, folding, antibody databases and data management systems, and ELN.
  • Knowledge of GLP/GMP requirements as they relate to CHO cell culture and cell line development.


Around the world, we are passionate about making an impact on the lives of patients with serious diseases. Empowered to apply our individual talents and diverse perspectives in an inclusive culture, our shared values of passion, innovation, urgency, accountability, inclusion and integrity bring out the highest potential of each of our colleagues.

Bristol Myers Squibb recognizes the importance of balance and flexibility in our work environment. We offer a wide variety of competitive benefits, services and programs that provide our employees with the resources to pursue their goals, both at work and in their personal lives.