Sr Associate, Process Development

Location
San Diego, CA, US
Posted
Sep 07, 2020
Ref
659548600
Hotbed
Biotech Beach
Required Education
Bachelors Degree
Position Type
Full time
Those who join Emergent BioSolutions feel a sense of ownership about their future. You will excel in an environment characterized by respect, innovation and growth opportunities. Here, you will join passionate professionals who advance their scientific, technical and professional skills to develop products designed-to protect life.

Job Summary

The primary focus for this position is mammalian cell culture, including but not limited to molecular cloning, cells freeze and thaw, media preparation, cell maintenance and passaging, scale up and bioreactor operation, transfection or infection, and cell harvest. Documentation of these activities is also required, including recording in lab notebooks, draft SOPs and batch records, writing development reports. Another focus for this position is cell line development related activities including molecular cloning, transfection, selection, single cell cloning and cell banking.

Essential Functions
  • Perform mammalian cell cultures in multi well plates, shake flasks, CellSTACKs and bioreactors. Prepare media and monitor cell density, viability etc.
  • Evaluate, develop and optimize the process parameters for upstream mammalian cell culture.
  • Operate related equipment.
  • Operate bioreactors, filters and AKTA.
  • Troubleshooting the issues related to cell culture process development.
  • Use DOE software to design the experiment and analyze the results.
  • Cell line development related activities including molecular cloning, transfection, selection, single cell cloning and cell banking.
  • Write technical reports and tech transfer reports , draft SOPs and batch records .
  • Work with vendors or CRO's to evaluate materials, equipment and to review development reports.
  • Travel may be required to support manufacture activities.


Minimum Education, Experience, Skills
  • B.S. in biology or related life science field with 2-5 years' relevant industry experience, or M.S. in molecular and cell biology or related life science field with at least 2 years' relevant industry experience.
  • Good aseptic technique is a must
  • Mammalian cell culture experience with various adherent cells and suspention cells such as Vero, HEK293, CHO cells is required.
  • Hand on experience with molecular cloning, cell cultrue and transfeciton is required.
  • Design of Experiment (DoE) experience is necessary .
  • Bioreactor (small scale and large scale) experience is preferred.
  • Background in Cell Biology, Molecular Biology or related discipline is preferred.
  • Engineering knowledge/hands on experience with equipment troubleshooting is preferred.
  • Experience with protein expression and detection by Western Blot and immunostaining is preferred.
  • Experience with quantitative and qualitative methods, including ELISA, MSD, fluorescence and IF microscopy, colorimetric, enzymatic assays and reporter assays, FACS assays is prefered.
  • Strong trouble shooting and data analysis skills.
  • Must be a team player and quick learner.
  • cGMP experience is preferred.
  • Good writing skills and communication skills.

Physical/Mental Demands and Work Environment Characteristics
  • There are physical/mental demands and work environment characteristics that must be met by an individual to successfully perform the essential functions of the job.
  • This information is available upon request from the candidate.
  • Reasonable accommodations may be made to enable individuals with disabilities to perform all essential functions.

Emergent BioSolutions is an Equal Opportunity/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, gender, age, national origin, disability, Protected Veteran status, sexual orientation, or any other characteristic protected by federal, state or local law.

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Emergent BioSolutions does not accept non-solicited resumes or candidate submittals from search/recruiting agencies not already on Emergent BioSolutions' approved agency list. Unsolicited resumes or candidate information submitted to Emergent BioSolutions by search/recruiting agencies not already on Emergent BioSolutions' approved agency list shall become the property of Emergent BioSolutions and if the candidate is subsequently hired by Emergent BioSolutions, Emergent BioSolutions shall not owe any fee to the submitting agency.