Scientist, Analytical Development

Location
Cambridge
Posted
Sep 06, 2020
Ref
4802085002
Hotbed
Genetown
Required Education
Doctorate/PHD/MD
Position Type
Full time
JOB PURPOSE

Sana Biotechnology is looking for a highly motivated scientist to join a dynamic, rapidly growing, and collaborative team in the Analytical Development Group. This individual will be at the forefront of gene therapy approaches utilizing a novel biological platform, and we are seeking a creative and hardworking individual that shares our excitement in the potential of this platform. He/she will drive the development of potency methods for our gene therapy programs and contribute to the analytical characterization of viral vectors for in-vitro research-based experiments. The candidate will execute assay development and testing activities as well as cell process efforts that lead to a robust and scalable manufacturing process. This position represents an excellent opportunity for career development, working closely with experienced cell and gene therapy scientists in a highly collaborative environment.

DUTIES AND RESPONSIBILITIES
  • Develop flow cytometry and MOA-reflective methodologies for use in both regulated (e.g. lot release, in-process, and stability testing) and unregulated environments (e.g. characterization, high throughput screening).
  • Drive potency assay development for gene therapy programs, including flow cytometry, ELISA, Luminex or MSD, and gene expression assays.
  • Develop vector characterization assays, such as sizing and particle aggregation
  • Develop, optimize, and execute robust and reproducible assays in support of both discovery research and pre-clinical development.
  • Integrate data with cross-functional R&D teams and present data regularly at team meetings.
  • Coordinate with various internal groups for evaluation of emerging vector products.
  • Implement superb documentation of procedures and experiments to support tech transfer.
  • Train and mentor junior associates.
  • Contribute to Sana's mission and culture.

QUALIFICATIONS

Basic Qualifications
  • PhD in immunology, cell/molecular biology, virology or related field scientific discipline and up to 2+ yrs academic or industry experience. BA and/or MS plus 7+ yrs or equivalent combination of education and work experience; Strong knowledge of required principals and concepts with understanding of context for application.
  • Strong knowledge of flow cytometry principles, method development & optimization and data analysis.
  • Familiarity with development and /or qualification of cell-based and gene therapy therapeutics, including the development of analytical methods.
  • Prior experience in development and execution of assays to characterize and measure proteins and DNA using a variety of methodologies including flow qPCR, ddPCR, electrophoresis, and ELISA.
  • Excellent bench skills with attention to detail and high standards for data integrity and quality.
  • Able to critically analyze data and troubleshoot methods and equipment.
  • The candidate should be a team player, highly organized with excellent communication skills, and demonstrate scientific rigor and excellent problem-solving skills.
  • Demonstrated effective management of scientists and/or research associates.

Preferred Qualifications
  • Experience with cell-based viral titration assays (e.g. flow cytometry, PCR, ELISA).
  • Experience with viral vector systems, production and/or purification.
  • Experience with liquid handler automation platforms (e.g. Integra, Hamilton, Tecan)
  • Familiarity with GxP principles and regulations as demonstrated by assay and/or technology transfer to GxP manufacturing, or significant laboratory support of IND/BLA submissions.
  • Experience in authoring and reviewing documentation including technical documents, protocols and reports connected to assay development, validation and sample testing in IND filing documents.

WORKING CONDITIONS
  • Flexibility to work some evenings or weekends in support of cell culture processing
  • Ability to travel

PHYSICAL REQUIREMENTS

Ability to perform assays, standard cell culture in a development lab.